caa a22 4500 605499322 CHVBK 20210128100550.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00253-014-6113-9 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6113-9 Denitrification of groundwater using a sulfur-oxidizing autotrophic denitrifying anaerobic fluidized-bed MBR: performance and bacterial community structure [Elektronische Daten] [Lili Zhang, Chao Zhang, Chengzhi Hu, Huijuan Liu, Jiuhui Qu] This paper investigates a novel sulfur-oxidizing autotrophic denitrifying anaerobic fluidized bed membrane bioreactor (AnFB-MBR) that has the potential to overcome the limitations of conventional sulfur-oxidizing autotrophic denitrification systems. The AnFB-MBR produced consistent high-quality product water when fed by a synthetic groundwater with NO3 −-N ranging 25-80mg/L and operated at hydraulic retention times of 0.5-5.0h. A nitrate removal rate of up to 4.0g NO3 −-N/Lreactord was attained by the bioreactor, which exceeded any reported removal capacity. The flux of AnFB-MBR was maintained in the range of 1.5-15Lm−2h−1. Successful membrane cleaning was practiced with cleaning cycles of 35-81days, which had no obvious effect on the AnFB-MBR performance. The 15 N-tracer analyses elucidated that nitrogen was converted into 15 N2-N and 15 N-biomass accounting for 88.1-93.1% and 6.4-11.6% of the total nitrogen produced, respectively. Only 0.3-0.5% of removed nitrogen was in form of 15N2O-N in sulfur-oxidizing autotrophic denitrification process, reducing potential risks of a significant amount of N2O emissions. The sulfur-oxidizing autotrophic denitrifying bacterial consortium was composed mainly of bacteria from Proteobacteria, Chlorobi, and Chloroflexi phyla, with genera Thiobacillus, Sulfurimonas, and Ignavibacteriales dominating the consortium. The pyrosequencing assays also suggested that the stable microbial communities corresponded to the elevated performance of the AnFB-MBR. Overall, this research described relatively high nitrate removal, acceptable flux, indicating future potential for the technology in practice. Springer-Verlag Berlin Heidelberg, 2014 Anaerobic fluidized bed membrane bioreactor nationallicence Sulfur-oxidizing autotrophic denitrification nationallicence Groundwater nationallicence Pyrosequencing nationallicence Microbial community nationallicence Zhang Lili Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 100085, Beijing, China aut Zhang Chao Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 100085, Beijing, China aut Hu Chengzhi Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 100085, Beijing, China aut Liu Huijuan Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 100085, Beijing, China aut Qu Jiuhui Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 100085, Beijing, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2815-2827 0175-7598 99:6<2815 2015 99 253 https://doi.org/10.1007/s00253-014-6113-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6113-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Lili Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 100085, Beijing, China aut NATIONALLICENCE 700 1- Zhang Chao Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 100085, Beijing, China aut NATIONALLICENCE 700 1- Hu Chengzhi Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 100085, Beijing, China aut NATIONALLICENCE 700 1- Liu Huijuan Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 100085, Beijing, China aut NATIONALLICENCE 700 1- Qu Jiuhui Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 100085, Beijing, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2815-2827 0175-7598 99:6<2815 2015 99 253