caa a22 4500 605499330 CHVBK 20210128100550.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00253-014-6155-z doi (NATIONALLICENCE)springer-10.1007/s00253-014-6155-z Using cassava distiller's dried grains as carbon and microbe sources to enhance denitrification of nitrate-contaminated groundwater [Elektronische Daten] [Rui Wan, Xiong Zheng, Yinguang Chen, Huaichen Wang] Nitrate removal from synthetic and real groundwater was investigated by using cassava distiller's dried grains (CDDG), which served as sole carbon source as well as the only microbe seed. It was found that remarkably higher total nitrogen removal efficiency (96.8±0.6%) could be reached; the accumulation of nitrite and the releases of organic compounds, meanwhile, were insignificant in the denitrification process. Scanning electron microscope (SEM) analysis showed that CDDG were degraded during the denitrification process. Further investigation showed that CDDG were anaerobically hydrolyzed and acidified to butyric acid, acetic acid, and carbohydrate, which could be utilized directly as the reducing equivalent providers for denitrification by the microorganisms separated from CDDG. Microbial community analysis revealed that the fungi and bacteria present in the original CDDG functioned as the denitrifiers, which mainly consisted of Aspergillus (69.8%) and Rhizomucor (15.9%) in the fungi community and Burkholderia (20.6%) and Rhizobium (15.9%) in the bacteria community, respectively. Finally, the use of CDDG as both carbon and microbial sources for real groundwater denitrification was testified to be feasible and safe with a total nitrogen removal efficiency of around 100%. Springer-Verlag Berlin Heidelberg, 2014 Denitrification nationallicence Carbon source nationallicence Microbe nationallicence Cassava distiller's dried grains nationallicence Groundwater nationallicence Wan Rui State Key Laboratory of Pollution Control and Resources Reuse, School of Environmental Science and Engineering, Tongji University, 1239 Siping Road, 200092, Shanghai, China aut Zheng Xiong State Key Laboratory of Pollution Control and Resources Reuse, School of Environmental Science and Engineering, Tongji University, 1239 Siping Road, 200092, Shanghai, China aut Chen Yinguang State Key Laboratory of Pollution Control and Resources Reuse, School of Environmental Science and Engineering, Tongji University, 1239 Siping Road, 200092, Shanghai, China aut Wang Huaichen State Key Laboratory of Pollution Control and Resources Reuse, School of Environmental Science and Engineering, Tongji University, 1239 Siping Road, 200092, Shanghai, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2839-2847 0175-7598 99:6<2839 2015 99 253 https://doi.org/10.1007/s00253-014-6155-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6155-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wan Rui State Key Laboratory of Pollution Control and Resources Reuse, School of Environmental Science and Engineering, Tongji University, 1239 Siping Road, 200092, Shanghai, China aut NATIONALLICENCE 700 1- Zheng Xiong State Key Laboratory of Pollution Control and Resources Reuse, School of Environmental Science and Engineering, Tongji University, 1239 Siping Road, 200092, Shanghai, China aut NATIONALLICENCE 700 1- Chen Yinguang State Key Laboratory of Pollution Control and Resources Reuse, School of Environmental Science and Engineering, Tongji University, 1239 Siping Road, 200092, Shanghai, China aut NATIONALLICENCE 700 1- Wang Huaichen State Key Laboratory of Pollution Control and Resources Reuse, School of Environmental Science and Engineering, Tongji University, 1239 Siping Road, 200092, Shanghai, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2839-2847 0175-7598 99:6<2839 2015 99 253