Enhancing the utilization of host trehalose by fungal trehalase improves the virulence of fungal insecticide
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6767-y |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6767-y | ||
| 245 | 0 | 0 | |a Enhancing the utilization of host trehalose by fungal trehalase improves the virulence of fungal insecticide |h [Elektronische Daten] |c [Guoxiong Peng, Kai Jin, Yingchun Liu, Yuxian Xia] |
| 520 | 3 | |a Entomopathogenic fungi proliferate in insect hemolymph by using host nutrients after penetrating the cuticle. To improve the virulence of the locust specific fungus, Metarhizium acridum, we genetically modified the fungus to overexpress ATM1, an endogenous hydrolase of trehalose, which is the main carbon source in insect hemolymph. Compared with the wild-type strain, Metarhizium acridum overexpressing ATM1 gene secreted more acid trehalase into locust hemolymph. The trehalose concentrations in locusts infected with the ATM1-overexpressing strain were 5.5 and 6.1mmol/l, lower than that in locusts infected with the wild-type strain at 3 and 5days post-inoculation, representing 44.5 and 60.7% reduction, respectively. Correspondingly, overexpressing ATM1 accelerated the growth of Metarhizium acridum in host hemolymph, and the dose causing 50% mortality (LD50) of the ATM1-overexpressing strain was reduced by 8.3-fold compared with the wild-type strain, suggesting that increasing the utilization of host nutrients by pathogens could be a promising way to improve the virulence of biopesticides based on parasites of pests. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Acid trehalase |2 nationallicence | |
| 690 | 7 | |a Metarhizium acridum |2 nationallicence | |
| 690 | 7 | |a Nutrient usage |2 nationallicence | |
| 690 | 7 | |a Growth |2 nationallicence | |
| 690 | 7 | |a Virulence |2 nationallicence | |
| 700 | 1 | |a Peng |D Guoxiong |u Genetic Engineering Research Center, School of Life Sciences, Chongqing University, 400045, Chongqing, People's Republic of China |4 aut | |
| 700 | 1 | |a Jin |D Kai |u Genetic Engineering Research Center, School of Life Sciences, Chongqing University, 400045, Chongqing, People's Republic of China |4 aut | |
| 700 | 1 | |a Liu |D Yingchun |u Genetic Engineering Research Center, School of Life Sciences, Chongqing University, 400045, Chongqing, People's Republic of China |4 aut | |
| 700 | 1 | |a Xia |D Yuxian |u Genetic Engineering Research Center, School of Life Sciences, Chongqing University, 400045, Chongqing, People's Republic of China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/20(2015-10-01), 8611-8618 |x 0175-7598 |q 99:20<8611 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6767-y |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6767-y |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Peng |D Guoxiong |u Genetic Engineering Research Center, School of Life Sciences, Chongqing University, 400045, Chongqing, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Jin |D Kai |u Genetic Engineering Research Center, School of Life Sciences, Chongqing University, 400045, Chongqing, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Liu |D Yingchun |u Genetic Engineering Research Center, School of Life Sciences, Chongqing University, 400045, Chongqing, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Xia |D Yuxian |u Genetic Engineering Research Center, School of Life Sciences, Chongqing University, 400045, Chongqing, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/20(2015-10-01), 8611-8618 |x 0175-7598 |q 99:20<8611 |1 2015 |2 99 |o 253 | ||