caa a22 4500 605499381 CHVBK 20210128100550.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00253-015-6767-y doi (NATIONALLICENCE)springer-10.1007/s00253-015-6767-y Enhancing the utilization of host trehalose by fungal trehalase improves the virulence of fungal insecticide [Elektronische Daten] [Guoxiong Peng, Kai Jin, Yingchun Liu, Yuxian Xia] Entomopathogenic fungi proliferate in insect hemolymph by using host nutrients after penetrating the cuticle. To improve the virulence of the locust specific fungus, Metarhizium acridum, we genetically modified the fungus to overexpress ATM1, an endogenous hydrolase of trehalose, which is the main carbon source in insect hemolymph. Compared with the wild-type strain, Metarhizium acridum overexpressing ATM1 gene secreted more acid trehalase into locust hemolymph. The trehalose concentrations in locusts infected with the ATM1-overexpressing strain were 5.5 and 6.1mmol/l, lower than that in locusts infected with the wild-type strain at 3 and 5days post-inoculation, representing 44.5 and 60.7% reduction, respectively. Correspondingly, overexpressing ATM1 accelerated the growth of Metarhizium acridum in host hemolymph, and the dose causing 50% mortality (LD50) of the ATM1-overexpressing strain was reduced by 8.3-fold compared with the wild-type strain, suggesting that increasing the utilization of host nutrients by pathogens could be a promising way to improve the virulence of biopesticides based on parasites of pests. Springer-Verlag Berlin Heidelberg, 2015 Acid trehalase nationallicence Metarhizium acridum nationallicence Nutrient usage nationallicence Growth nationallicence Virulence nationallicence Peng Guoxiong Genetic Engineering Research Center, School of Life Sciences, Chongqing University, 400045, Chongqing, People's Republic of China aut Jin Kai Genetic Engineering Research Center, School of Life Sciences, Chongqing University, 400045, Chongqing, People's Republic of China aut Liu Yingchun Genetic Engineering Research Center, School of Life Sciences, Chongqing University, 400045, Chongqing, People's Republic of China aut Xia Yuxian Genetic Engineering Research Center, School of Life Sciences, Chongqing University, 400045, Chongqing, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/20(2015-10-01), 8611-8618 0175-7598 99:20<8611 2015 99 253 https://doi.org/10.1007/s00253-015-6767-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6767-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Peng Guoxiong Genetic Engineering Research Center, School of Life Sciences, Chongqing University, 400045, Chongqing, People's Republic of China aut NATIONALLICENCE 700 1- Jin Kai Genetic Engineering Research Center, School of Life Sciences, Chongqing University, 400045, Chongqing, People's Republic of China aut NATIONALLICENCE 700 1- Liu Yingchun Genetic Engineering Research Center, School of Life Sciences, Chongqing University, 400045, Chongqing, People's Republic of China aut NATIONALLICENCE 700 1- Xia Yuxian Genetic Engineering Research Center, School of Life Sciences, Chongqing University, 400045, Chongqing, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/20(2015-10-01), 8611-8618 0175-7598 99:20<8611 2015 99 253