caa a22 4500 60549939X CHVBK 20210128100550.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00253-015-6734-7 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6734-7 Fine-tuning of ecaA and pepc gene expression increases succinic acid production in Escherichia coli [Elektronische Daten] [Jing Wang, Dandan Qin, Baoyun Zhang, Qiang Li, Sha Li, Xiaohua Zhou, Lichun Dong, Dan Wang] For making a complex synthetic gene network function as designed, the parameters in the network have to be extensively tuned. In this study, a simple and general approach to rapidly tune gene networks in Escherichia coli was developed, which uses the hypermutable simple sequence repeats embedded in the spacer region between the ribosome binding site and the initiation codon. It was found that the change of sequence length and compositions of the repeated base pairs in 5′UTR contributes together to the changeable expression levels of the target gene. The mechanism of this phenomenon is that the transcriptional process makes greater impact on the expression level when compared to the translational process, which is utilized to successfully predict sample gene expression levels over a 50-fold range. The utility of the approach to regulate heterologous ecaA and pepc gene expression in the engineered E. coli for improving succinic acid yield and production has also been demonstrated. When the expression level of ecaA gene was 3.53-fold of the control and the expression level of pepc gene was 1.06-fold of the control, the highest yield of succinic acid and productivity were achieved, which was 0.87gg−1 and 2.01gL−1h−1, respectively. Springer-Verlag Berlin Heidelberg, 2015 Gene regulation nationallicence RNA polymerase holoenzyme nationallicence RBS nationallicence Transcriptional level nationallicence Translational level nationallicence Succinic acid nationallicence Wang Jing School of Chemistry and Chemical Engineering, Chongqing University, 400044, Chongqing, China aut Qin Dandan School of Chemistry and Chemical Engineering, Chongqing University, 400044, Chongqing, China aut Zhang Baoyun School of Bioengineering, Chongqing University, 400044, Chongqing, China aut Li Qiang National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100190, Beijing, China aut Li Sha State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing University of Technology, 210009, Nanjing, China aut Zhou Xiaohua School of Chemistry and Chemical Engineering, Chongqing University, 400044, Chongqing, China aut Dong Lichun School of Chemistry and Chemical Engineering, Chongqing University, 400044, Chongqing, China aut Wang Dan School of Chemistry and Chemical Engineering, Chongqing University, 400044, Chongqing, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/20(2015-10-01), 8575-8586 0175-7598 99:20<8575 2015 99 253 https://doi.org/10.1007/s00253-015-6734-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6734-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Jing School of Chemistry and Chemical Engineering, Chongqing University, 400044, Chongqing, China aut NATIONALLICENCE 700 1- Qin Dandan School of Chemistry and Chemical Engineering, Chongqing University, 400044, Chongqing, China aut NATIONALLICENCE 700 1- Zhang Baoyun School of Bioengineering, Chongqing University, 400044, Chongqing, China aut NATIONALLICENCE 700 1- Li Qiang National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100190, Beijing, China aut NATIONALLICENCE 700 1- Li Sha State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing University of Technology, 210009, Nanjing, China aut NATIONALLICENCE 700 1- Zhou Xiaohua School of Chemistry and Chemical Engineering, Chongqing University, 400044, Chongqing, China aut NATIONALLICENCE 700 1- Dong Lichun School of Chemistry and Chemical Engineering, Chongqing University, 400044, Chongqing, China aut NATIONALLICENCE 700 1- Wang Dan School of Chemistry and Chemical Engineering, Chongqing University, 400044, Chongqing, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/20(2015-10-01), 8575-8586 0175-7598 99:20<8575 2015 99 253