Molecular characterization of a Penicillium chrysogenum exo-rhamnogalacturonan lyase that is structurally distinct from other polysaccharide lyase family proteins
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6600-7 |2 doi |
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| 245 | 0 | 0 | |a Molecular characterization of a Penicillium chrysogenum exo-rhamnogalacturonan lyase that is structurally distinct from other polysaccharide lyase family proteins |h [Elektronische Daten] |c [Marin Iwai, Takuya Kawakami, Takeshi Ikemoto, Daisuke Fujiwara, Shigeo Takenaka, Masami Nakazawa, Mitsuhiro Ueda, Tatsuji Sakamoto] |
| 520 | 3 | |a We previously described an endo-acting rhamnogalacturonan (RG) lyase, termed PcRGL4A, of Penicillium chrysogenum 31B. Here, we describe a second RG lyase, called PcRGLX. We determined the cDNA sequence of the Pcrglx gene, which encodes PcRGLX. Based on analyses using a BLAST search and a conserved domain search, PcRGLX was found to be structurally distinct from known RG lyases and might belong to a new polysaccharide lyase family together with uncharacterized fungal proteins of Nectria haematococca, Aspergillus oryzae, and Fusarium oxysporum. The Pcrglx cDNA gene product (rPcRGLX) expressed in Escherichia coli demonstrated specific activity against RG but not against homogalacturonan. Divalent cations were not essential for the enzymatic activity of rPcRGLX. rPcRGLX mainly released unsaturated galacturonosyl rhamnose (ΔGR) from RG backbones used as the substrate from the initial stage of the reaction, indicating that the enzyme can be classified as an exo-acting RG lyase (EC 4.2.2.24). This is the first report of an RG lyase with this mode of action in Eukaryota. rPcRGLX acted synergistically with PcRGL4A to degrade soybean RG and released ΔGR. This ΔGR was partially decorated with galactose (Gal) residues, indicating that rPcRGLX preferred oligomeric RGs to polymeric RGs, that the enzyme did not require Gal decoration of RG backbones for degradation, and that the enzyme bypassed the Gal side chains of RG backbones. These characteristics of rPcRGLX might be useful in the determination of complex structures of pectins. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Exo-acting rhamnogalacturonan lyase |2 nationallicence | |
| 690 | 7 | |a Nucleotide sequence |2 nationallicence | |
| 690 | 7 | |a Overexpression |2 nationallicence | |
| 690 | 7 | |a Penicillium chrysogenum |2 nationallicence | |
| 690 | 7 | |a Unsaturated galacturonosyl rhamnose |2 nationallicence | |
| 700 | 1 | |a Iwai |D Marin |u Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 599-8531, Sakai, Osaka, Japan |4 aut | |
| 700 | 1 | |a Kawakami |D Takuya |u Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 599-8531, Sakai, Osaka, Japan |4 aut | |
| 700 | 1 | |a Ikemoto |D Takeshi |u Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 599-8531, Sakai, Osaka, Japan |4 aut | |
| 700 | 1 | |a Fujiwara |D Daisuke |u Department of Biological Science, Graduate School of Science, Osaka Prefecture University, 599-8531, Sakai, Osaka, Japan |4 aut | |
| 700 | 1 | |a Takenaka |D Shigeo |u Division of Veterinary science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 598-8531, Izumisano, Osaka, Japan |4 aut | |
| 700 | 1 | |a Nakazawa |D Masami |u Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 599-8531, Sakai, Osaka, Japan |4 aut | |
| 700 | 1 | |a Ueda |D Mitsuhiro |u Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 599-8531, Sakai, Osaka, Japan |4 aut | |
| 700 | 1 | |a Sakamoto |D Tatsuji |u Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 599-8531, Sakai, Osaka, Japan |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/20(2015-10-01), 8515-8525 |x 0175-7598 |q 99:20<8515 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6600-7 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6600-7 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Iwai |D Marin |u Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 599-8531, Sakai, Osaka, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kawakami |D Takuya |u Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 599-8531, Sakai, Osaka, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ikemoto |D Takeshi |u Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 599-8531, Sakai, Osaka, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Fujiwara |D Daisuke |u Department of Biological Science, Graduate School of Science, Osaka Prefecture University, 599-8531, Sakai, Osaka, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Takenaka |D Shigeo |u Division of Veterinary science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 598-8531, Izumisano, Osaka, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Nakazawa |D Masami |u Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 599-8531, Sakai, Osaka, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ueda |D Mitsuhiro |u Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 599-8531, Sakai, Osaka, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sakamoto |D Tatsuji |u Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 599-8531, Sakai, Osaka, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/20(2015-10-01), 8515-8525 |x 0175-7598 |q 99:20<8515 |1 2015 |2 99 |o 253 | ||