Production of phenylpyruvic acid from l -phenylalanine using an l -amino acid deaminase from Proteus mirabilis
comparison of enzymatic and whole-cell biotransformation approaches
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6757-0 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6757-0 | ||
| 245 | 0 | 0 | |a Production of phenylpyruvic acid from l -phenylalanine using an l -amino acid deaminase from Proteus mirabilis |h [Elektronische Daten] |b comparison of enzymatic and whole-cell biotransformation approaches |c [Ying Hou, Gazi Hossain, Jianghua Li, Hyun-dong Shin, Long Liu, Guocheng Du] |
| 520 | 3 | |a Phenylpyruvic acid (PPA) is an important organic acid that has a wide range of applications. In this study, the membrane-bound l-amino acid deaminase (l-AAD) gene from Proteus mirabilis KCTC 2566 was expressed in Escherichia coli BL21(DE3) and then the l-AAD was purified. After that, we used the purified enzyme and the recombinant E. coli whole-cell biocatalyst to produce PPA via a one-step biotransformation from l-phenylalanine. l-AAD was solubilized from the membrane and purified 52-fold with an overall yield of 13%, which corresponded to a specific activity of 0.94 ± 0.01μmol PPA min−1·mg−1. Then, the biotransformation conditions for the pure enzyme and the whole-cell biocatalyst were optimized. The maximal production was 2.6 ± 0.1g·L−1 (specific activity of 1.02 ± 0.02μmol PPA min−1·mg−1 protein, 86.7 ± 5% mass conversion rate, and 1.04g·L−1·h−1 productivity) and 3.3 ± 0.2gL−1 (specific activity of 0.013 ± 0.003μmol PPA min−1·mg−1 protein, 82.5 ± 4% mass conversion rate, and 0.55g·L−1·h−1 productivity) for the pure enzyme and whole-cell biocatalyst, respectively. Comparative studies of the enzymatic and whole-cell biotransformation were performed in terms of specific activity, production, conversion, productivity, stability, need of external cofactors, and recycling. We have developed two eco-friendly and efficient approaches for PPA production. The strategy described herein may aid the biotransformational synthesis of other α-keto acids from their corresponding amino acids. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a l -Amino acid deaminase |2 nationallicence | |
| 690 | 7 | |a Phenylpyruvic acid |2 nationallicence | |
| 690 | 7 | |a Proteus mirabilis |2 nationallicence | |
| 690 | 7 | |a Whole-cell biotransformation |2 nationallicence | |
| 700 | 1 | |a Hou |D Ying |u Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China |4 aut | |
| 700 | 1 | |a Hossain |D Gazi |u Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China |4 aut | |
| 700 | 1 | |a Li |D Jianghua |u Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China |4 aut | |
| 700 | 1 | |a Shin |D Hyun-dong |u School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 30332, Atlanta, USA |4 aut | |
| 700 | 1 | |a Liu |D Long |u Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China |4 aut | |
| 700 | 1 | |a Du |D Guocheng |u Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/20(2015-10-01), 8391-8402 |x 0175-7598 |q 99:20<8391 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6757-0 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6757-0 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Hou |D Ying |u Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Hossain |D Gazi |u Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Li |D Jianghua |u Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Shin |D Hyun-dong |u School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 30332, Atlanta, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Liu |D Long |u Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Du |D Guocheng |u Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/20(2015-10-01), 8391-8402 |x 0175-7598 |q 99:20<8391 |1 2015 |2 99 |o 253 | ||