caa a22 4500 605499489 CHVBK 20210128100551.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00253-015-6864-y doi (NATIONALLICENCE)springer-10.1007/s00253-015-6864-y Isolation and characterisation of non-anaerobic butanol-producing symbiotic system TSH06 [Elektronische Daten] [Genyu Wang, Pengfei Wu, Ya Liu, Shuo Mi, Shuai Mai, Chunkai Gu, Gehua Wang, Hongjuan Liu, Jianan Zhang, Børre Børresen, Evy Mellemsæther, Hans Kotlar] Butanol-producing microorganisms are all obligate anaerobes. In this study, a unique symbiotic system TSH06 was isolated to be capable of producing butanol under non-anaerobic condition. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S ribosomal RNA (rRNA) revealed that two strains coexist in TSH06. The two strains were identical to Clostridium acetobutylicum and Bacillus cereus, respectively. They were isolated individually and named as C. acetobutylicum TSH1 and B. cereus TSH2. C. acetobutylicum TSH1 is a butanol-producing, obligate anaerobic strain. Facultative anaerobic B. cereus TSH2 did not possess the ability of butanol production; however, it offered C. acetobutylicum TSH1 the viability under non-anaerobic condition. Moreover, B. cereus TSH2 enhanced butanol yield and speed of fermentation. TSH06 produced 12.97g/L butanol and 15.39g/L total solvent under non-anaerobic condition, which is 25 and 24%, respectively, higher than those of C. acetobutylicum TSH1. In addition, TSH06 produced butanol faster under non-anaerobic condition than under anaerobic condition. Butanol accounted for more than 80% of total solvent, which is higher than the known report. TSH06 was stable during passage. In all, TSH06 is a promising candidate for industrialisation of biobutanol with high yield, high butanol proportion, easy-handling and time-saving system. These results demonstrated the potential advantage of symbiosis. This study also provides a promising strategy for butanol fermentation. Springer-Verlag Berlin Heidelberg, 2015 Butanol nationallicence Biofuel nationallicence Symbiosis nationallicence TSH06 nationallicence Non-anaerobic fermentation nationallicence Wang Genyu Institute of Nuclear and New Energy Technology, Tsinghua University, 100084, Beijing, China aut Wu Pengfei Institute of Nuclear and New Energy Technology, Tsinghua University, 100084, Beijing, China aut Liu Ya Institute of Nuclear and New Energy Technology, Tsinghua University, 100084, Beijing, China aut Mi Shuo Institute of Nuclear and New Energy Technology, Tsinghua University, 100084, Beijing, China aut Mai Shuai Institute of Nuclear and New Energy Technology, Tsinghua University, 100084, Beijing, China aut Gu Chunkai Institute of Nuclear and New Energy Technology, Tsinghua University, 100084, Beijing, China aut Wang Gehua Institute of Nuclear and New Energy Technology, Tsinghua University, 100084, Beijing, China aut Liu Hongjuan Institute of Nuclear and New Energy Technology, Tsinghua University, 100084, Beijing, China aut Zhang Jianan Institute of Nuclear and New Energy Technology, Tsinghua University, 100084, Beijing, China aut Børresen Børre Statoil Petroleum AS, N-4035, Stavanger, Norway aut Mellemsæther Evy Statoil Petroleum AS, N-4035, Stavanger, Norway aut Kotlar Hans Statoil Petroleum AS, N-4035, Stavanger, Norway aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/20(2015-10-01), 8803-8813 0175-7598 99:20<8803 2015 99 253 https://doi.org/10.1007/s00253-015-6864-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6864-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Genyu Institute of Nuclear and New Energy Technology, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Wu Pengfei Institute of Nuclear and New Energy Technology, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Liu Ya Institute of Nuclear and New Energy Technology, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Mi Shuo Institute of Nuclear and New Energy Technology, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Mai Shuai Institute of Nuclear and New Energy Technology, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Gu Chunkai Institute of Nuclear and New Energy Technology, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Wang Gehua Institute of Nuclear and New Energy Technology, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Liu Hongjuan Institute of Nuclear and New Energy Technology, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Zhang Jianan Institute of Nuclear and New Energy Technology, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Børresen Børre Statoil Petroleum AS, N-4035, Stavanger, Norway aut NATIONALLICENCE 700 1- Mellemsæther Evy Statoil Petroleum AS, N-4035, Stavanger, Norway aut NATIONALLICENCE 700 1- Kotlar Hans Statoil Petroleum AS, N-4035, Stavanger, Norway aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/20(2015-10-01), 8803-8813 0175-7598 99:20<8803 2015 99 253