caa a22 4500 605499500 CHVBK 20210128100551.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00253-015-6702-2 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6702-2 Modulation of the intestinal microbiota and morphology of tilapia, Oreochromis niloticus , following the application of a multi-species probiotic [Elektronische Daten] [B Standen, A. Rodiles, D. Peggs, S. Davies, G. Santos, D. Merrifield] The intestinal microbiota and morphology of tilapia (Oreochromis niloticus) were investigated after the application of a multi-species probiotic containing Lactobacillus reuteri, Bacillus subtilis, Enterococcus faecium and Pediococcus acidilactici (AquaStar® Growout). Tilapia (55.03±0.44g) were fed either a control diet or a probiotic diet (control diet supplemented with AquaStar® Growout at 5gkg−1). After four and eightweeks, culture-dependent analysis showed higher levels of lactic acid bacteria (LAB), enterococci and Bacillus spp. in the mucosa and digesta of fish fed AquaStar® Growout. At week four, polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) revealed a higher similarity within the probiotic fed replicates than replicates of the control group; after eightweeks, the compositional dissimilarity of the microbiome profiles between the groups was greater than the dissimilarities within each group (P<0.05). High-throughput sequencing revealed that the probiotic treatment significantly reduced the number of operational taxonomic units and species richness in the digesta. Significantly higher proportions of reads belonging to Proteobacteria and Cyanobacteria were detected in the control group whereas the probiotic-fed fish displayed a significantly higher abundance of reads assigned to the Firmicutes (which accounted for >99% of reads). Bacillus, Cetobacterium and Mycobacterium were the dominant genera in the digesta of control fish whereas Bacillus, Enterococcus and Pediococcus were the largest constituents in probiotic-fed fish. The addition of AquaStar® Growout to tilapia diets led to increased populations of intraepithelial leucocytes, a higher absorptive surface area index and higher microvilli density in the intestine. These data suggest that AquaStar® Growout can modulate both the intestinal microbiota and morphology of tilapia. Springer-Verlag Berlin Heidelberg, 2015 Probiotic nationallicence Intestinal microbiota nationallicence High-throughput sequencing nationallicence Intraepithelial leucocyte (IEL) nationallicence Microscopy nationallicence Tilapia nationallicence Standen B. Aquaculture and Fish Nutrition Research Group, School of Biological Sciences, Plymouth University, Drake Circus, PL4 8AA, Plymouth, Devon, UK aut Rodiles A. Aquaculture and Fish Nutrition Research Group, School of Biological Sciences, Plymouth University, Drake Circus, PL4 8AA, Plymouth, Devon, UK aut Peggs D. Aquaculture and Fish Nutrition Research Group, School of Biological Sciences, Plymouth University, Drake Circus, PL4 8AA, Plymouth, Devon, UK aut Davies S. Aquaculture and Fish Nutrition Research Group, School of Biological Sciences, Plymouth University, Drake Circus, PL4 8AA, Plymouth, Devon, UK aut Santos G. Biomin Holding GmbH, Industriestrasse 21, 3130, Herzogenburg, Austria aut Merrifield D. Aquaculture and Fish Nutrition Research Group, School of Biological Sciences, Plymouth University, Drake Circus, PL4 8AA, Plymouth, Devon, UK aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/20(2015-10-01), 8403-8417 0175-7598 99:20<8403 2015 99 253 https://doi.org/10.1007/s00253-015-6702-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6702-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Standen B. Aquaculture and Fish Nutrition Research Group, School of Biological Sciences, Plymouth University, Drake Circus, PL4 8AA, Plymouth, Devon, UK aut NATIONALLICENCE 700 1- Rodiles A. Aquaculture and Fish Nutrition Research Group, School of Biological Sciences, Plymouth University, Drake Circus, PL4 8AA, Plymouth, Devon, UK aut NATIONALLICENCE 700 1- Peggs D. Aquaculture and Fish Nutrition Research Group, School of Biological Sciences, Plymouth University, Drake Circus, PL4 8AA, Plymouth, Devon, UK aut NATIONALLICENCE 700 1- Davies S. Aquaculture and Fish Nutrition Research Group, School of Biological Sciences, Plymouth University, Drake Circus, PL4 8AA, Plymouth, Devon, UK aut NATIONALLICENCE 700 1- Santos G. Biomin Holding GmbH, Industriestrasse 21, 3130, Herzogenburg, Austria aut NATIONALLICENCE 700 1- Merrifield D. Aquaculture and Fish Nutrition Research Group, School of Biological Sciences, Plymouth University, Drake Circus, PL4 8AA, Plymouth, Devon, UK aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/20(2015-10-01), 8403-8417 0175-7598 99:20<8403 2015 99 253