caa a22 4500 605499535 CHVBK 20210128100551.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00253-015-6707-x doi (NATIONALLICENCE)springer-10.1007/s00253-015-6707-x Isolation of phenazine 1,6-di-carboxylic acid from Pseudomonas aeruginosa strain HRW.1-S3 and its role in biofilm-mediated crude oil degradation and cytotoxicity against bacterial and cancer cells [Elektronische Daten] [Debdeep Dasgupta, Abhinash Kumar, Balaram Mukhopadhyay, Tapas Sengupta] Pseudomonas sp. has long been known for production of a wide range of secondary metabolites during late exponential and stationary phases of growth. Phenazine derivatives constitute a large group of secondary metabolites produced by microorganisms including Pseudomonas sp. Phenazine 1,6-di-carboxylic acid (PDC) is one of such metabolites and has been debated for its origin from Pseudomonas sp. The present study describes purification and characterization of PDC isolated from culture of a natural isolate of Pseudomonas sp. HRW.1-S3 while grown in presence of crude oil as sole carbon source. The isolated PDC was tested for its effect on biofilm formation by another environmental isolate of Pseudomonas sp. DSW.1-S4 which lacks the ability to produce any phenazine compound. PDC showed profound effect on both planktonic as well as biofilm mode of growth of DSW.1-S4 at concentrations between 5 and 20μM. Interestingly, PDC showed substantial cytotoxicity against three cancer cell lines and against both Gram-positive and Gram-negative bacteria. Thus, the present study not only opens an avenue to understand interspecific cooperation between Pseudomonas species which may lead its applicability in bioremediation, but also it signifies the scope of future investigation on PDC for its therapeutic applications. Springer-Verlag Berlin Heidelberg, 2015 Pseudomonas nationallicence Phenazine 1,6-di-carboxylic acid nationallicence Biofilm nationallicence Skewness nationallicence Oil degradation nationallicence Cytotoxicity nationallicence Dasgupta Debdeep Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Kolkata, 741246, Mohanpur, India aut Kumar Abhinash Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Kolkata, 741246, Mohanpur, India aut Mukhopadhyay Balaram Department of Chemical Sciences, Indian Institute of Science Education and Research (IISER) Kolkata, 741246, Mohanpur, India aut Sengupta Tapas Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Kolkata, 741246, Mohanpur, India aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/20(2015-10-01), 8653-8665 0175-7598 99:20<8653 2015 99 253 https://doi.org/10.1007/s00253-015-6707-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6707-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dasgupta Debdeep Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Kolkata, 741246, Mohanpur, India aut NATIONALLICENCE 700 1- Kumar Abhinash Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Kolkata, 741246, Mohanpur, India aut NATIONALLICENCE 700 1- Mukhopadhyay Balaram Department of Chemical Sciences, Indian Institute of Science Education and Research (IISER) Kolkata, 741246, Mohanpur, India aut NATIONALLICENCE 700 1- Sengupta Tapas Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Kolkata, 741246, Mohanpur, India aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/20(2015-10-01), 8653-8665 0175-7598 99:20<8653 2015 99 253