caa a22 4500 605499543 CHVBK 20210128100551.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00253-015-6737-4 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6737-4 Designing and creating Saccharomyces interspecific hybrids for improved, industry relevant, phenotypes [Elektronische Daten] [Jennifer Bellon, Fei Yang, Martin Day, Debra Inglis, Paul Chambers] To remain competitive in increasingly overcrowded markets, yeast strain development programmes are crucial for fermentation-based food and beverage industries. In a winemaking context, there are many yeast phenotypes that stand to be improved. For example, winemakers endeavouring to produce sweet dessert wines wrestle with fermentation challenges particular to fermenting high-sugar juices, which can lead to elevated volatile acidity levels and extended fermentation times. In the current study, we used natural yeast breeding techniques to generate Saccharomyces spp. interspecific hybrids as a non-genetically modified (GM) strategy to introduce targeted improvements in important, wine-relevant traits. The hybrids were generated by mating a robust wine strain of Saccharomyces cerevisiae with a wine isolate of Saccharomyces bayanus, a species previously reported to produce wines with low concentrations of acetic acid. Two hybrids generated from the cross showed robust fermentation properties in high-sugar grape juice and produced botrytised Riesling wines with much lower concentrations of acetic acid relative to the industrial wine yeast parent. The hybrids also displayed suitability for icewine production when bench-marked against an industry standard icewine yeast, by delivering icewines with lower levels of acetic acid. Additionally, the hybrid yeast produced wines with novel aroma and flavour profiles and established that choice of yeast strain impacts on wine colour. These new hybrid yeasts display the desired targeted fermentation phenotypes from both parents, robust fermentation in high-sugar juice and the production of wines with low volatile acidity, thus establishing their suitability for wine styles that are traditionally troubled by excessive volatile acidity levels. Springer-Verlag Berlin Heidelberg, 2015 Saccharomyces interspecific hybrids nationallicence Targeted wine yeast strain development nationallicence Non-genetically modified (non-GM) nationallicence High-sugar fermentation nationallicence Bellon Jennifer The Australian Wine Research Institute, PO Box 197, 506, Glen Osmond, SA, Australia aut Yang Fei Cool Climate Oenology and Viticulture Institute, Brock University, St Catharines, ON, Canada aut Day Martin The Australian Wine Research Institute, PO Box 197, 506, Glen Osmond, SA, Australia aut Inglis Debra Cool Climate Oenology and Viticulture Institute, Brock University, St Catharines, ON, Canada aut Chambers Paul The Australian Wine Research Institute, PO Box 197, 506, Glen Osmond, SA, Australia aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/20(2015-10-01), 8597-8609 0175-7598 99:20<8597 2015 99 253 https://doi.org/10.1007/s00253-015-6737-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6737-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bellon Jennifer The Australian Wine Research Institute, PO Box 197, 506, Glen Osmond, SA, Australia aut NATIONALLICENCE 700 1- Yang Fei Cool Climate Oenology and Viticulture Institute, Brock University, St Catharines, ON, Canada aut NATIONALLICENCE 700 1- Day Martin The Australian Wine Research Institute, PO Box 197, 506, Glen Osmond, SA, Australia aut NATIONALLICENCE 700 1- Inglis Debra Cool Climate Oenology and Viticulture Institute, Brock University, St Catharines, ON, Canada aut NATIONALLICENCE 700 1- Chambers Paul The Australian Wine Research Institute, PO Box 197, 506, Glen Osmond, SA, Australia aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/20(2015-10-01), 8597-8609 0175-7598 99:20<8597 2015 99 253