Highly efficient biosynthesis of astaxanthin in Saccharomyces cerevisiae by integration and tuning of algal crtZ and bkt
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6791-y |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6791-y | ||
| 245 | 0 | 0 | |a Highly efficient biosynthesis of astaxanthin in Saccharomyces cerevisiae by integration and tuning of algal crtZ and bkt |h [Elektronische Daten] |c [Pingping Zhou, Lidan Ye, Wenping Xie, Xiaomei Lv, Hongwei Yu] |
| 520 | 3 | |a Astaxanthin is a highly valued carotenoid with strong antioxidant activity and has wide applications in aquaculture, food, cosmetic, and pharmaceutical industries. The market demand for natural astaxanthin promotes research in metabolic engineering of heterologous hosts for astaxanthin production. In this study, an astaxanthin-producing Saccharomyces cerevisiae strain was created by successively introducing the Haematococcus pluvialis β-carotenoid hydroxylase (crtZ) and ketolase (bkt) genes into a previously constructed β-carotene hyperproducer. Further integration of strategies including codon optimization, gene copy number adjustment, and iron cofactor supplementation led to significant increase in the astaxanthin production, reaching up to 4.7mg/g DCW in the shake-flask cultures which is the highest astaxanthin content in S. cerevisiae reported to date. Besides, the substrate specificity of H. pluvialis CrtZ and BKT and the probable formation route of astaxanthin from β-carotene in S. cerevisiae were figured out by expressing the genes separately and in combination. The yeast strains engineered in this work provide a basis for further improving biotechnological production of astaxanthin and might offer a useful general approach to the construction of heterologous biosynthetic pathways for other natural products. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Astaxanthin |2 nationallicence | |
| 690 | 7 | |a Metabolic engineering |2 nationallicence | |
| 690 | 7 | |a β-Carotenoid hydroxylase |2 nationallicence | |
| 690 | 7 | |a β-Carotenoid ketolase |2 nationallicence | |
| 690 | 7 | |a Saccharomyces cerevisiae |2 nationallicence | |
| 690 | 7 | |a Haematococcus pluvialis |2 nationallicence | |
| 700 | 1 | |a Zhou |D Pingping |u Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, 310027, Hangzhou, People's Republic of China |4 aut | |
| 700 | 1 | |a Ye |D Lidan |u Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, 310027, Hangzhou, People's Republic of China |4 aut | |
| 700 | 1 | |a Xie |D Wenping |u Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, 310027, Hangzhou, People's Republic of China |4 aut | |
| 700 | 1 | |a Lv |D Xiaomei |u Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, 310027, Hangzhou, People's Republic of China |4 aut | |
| 700 | 1 | |a Yu |D Hongwei |u Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, 310027, Hangzhou, People's Republic of China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/20(2015-10-01), 8419-8428 |x 0175-7598 |q 99:20<8419 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6791-y |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6791-y |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhou |D Pingping |u Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, 310027, Hangzhou, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ye |D Lidan |u Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, 310027, Hangzhou, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Xie |D Wenping |u Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, 310027, Hangzhou, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lv |D Xiaomei |u Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, 310027, Hangzhou, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yu |D Hongwei |u Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, 310027, Hangzhou, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/20(2015-10-01), 8419-8428 |x 0175-7598 |q 99:20<8419 |1 2015 |2 99 |o 253 | ||