caa a22 4500 605499578 CHVBK 20210128100551.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00253-015-6724-9 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6724-9 Experimental application of Lactobacillus fermentum CCM 7421 in combination with chlorophyllin in dogs [Elektronische Daten] [Viola Strompfová, Ivana Kubašová, Jana Farbáková, Soňa Gancarčíková, Dagmar Mudroňová, Aladár Maďari, Andrea Lauková] Chlorophyll belongs in a larger class of phytochemical plant pigments currently receiving more attention as a physiologically active dietary component. Although most research has focused on its biological activities such as its antioxidant, antimutagenic, anti-inflammatory or apoptotic effects in humans or rodents, there is limited knowledge at this time about the combinative possibilities of chlorophyll with probiotic bacteria. Our aim was to test the growth characteristics of canine-derived probiotic strain Lactobacillus fermentum CCM 7421 in the presence of different concentrations of chlorophyllin in vitro. Antimicrobial activity of chlorophyllin against canine indicator bacteria was also detected. In the in vivo study, chlorophyllin, L. fermentum CCM 7421 and the combination of both additives on faecal microbiota, faecal organic acid concentrations, haematological and immunological parameters in dogs were tested. Forty dogs were divided into 4 treatment groups; control (C); receiving chlorophyllin (60mg/day/dog, CH group); L. fermentum CCM 7421 (108CFU/day/dog, LF group); and both additives (CH+LF group), 10 dogs in each group. The experiment lasted for 28days with a 14-day treatment period (sample collection at days 0, 7, 14 and 28). Results showed no growth inhibition of strain CCM 7421 by 0.05-0.25% of chlorophyllin in broth after 24h. Reduced growth of staphylococci, Listeria monocytogenes and Citrobacter freundii was observed at 1% chlorophyllin (P<0.05). In dogs, lower coliform bacteria numbers and higher concentration of propionic acid in faeces of the CH group during the treatment compared to baseline were detected (P<0.01). Phagocytic activity of leukocytes was stimulated in all three treated groups of dogs (P<0.05). Springer-Verlag Berlin Heidelberg, 2015 Chlorophyll nationallicence Probiotic nationallicence Lactobacillus nationallicence Dogs nationallicence Intestinal microbiota nationallicence Haematology nationallicence Strompfová Viola Institute of Animal Physiology Slovak Academy of Sciences, Šoltésovej 4-6, 040 01, Košice, Slovakia aut Kubašová Ivana Institute of Animal Physiology Slovak Academy of Sciences, Šoltésovej 4-6, 040 01, Košice, Slovakia aut Farbáková Jana Small Animal Clinic, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81, Košice, Slovakia aut Gancarčíková Soňa Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81, Košice, Slovakia aut Mudroňová Dagmar Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81, Košice, Slovakia aut Maďari Aladár Small Animal Clinic, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81, Košice, Slovakia aut Lauková Andrea Institute of Animal Physiology Slovak Academy of Sciences, Šoltésovej 4-6, 040 01, Košice, Slovakia aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/20(2015-10-01), 8681-8690 0175-7598 99:20<8681 2015 99 253 https://doi.org/10.1007/s00253-015-6724-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6724-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Strompfová Viola Institute of Animal Physiology Slovak Academy of Sciences, Šoltésovej 4-6, 040 01, Košice, Slovakia aut NATIONALLICENCE 700 1- Kubašová Ivana Institute of Animal Physiology Slovak Academy of Sciences, Šoltésovej 4-6, 040 01, Košice, Slovakia aut NATIONALLICENCE 700 1- Farbáková Jana Small Animal Clinic, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81, Košice, Slovakia aut NATIONALLICENCE 700 1- Gancarčíková Soňa Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81, Košice, Slovakia aut NATIONALLICENCE 700 1- Mudroňová Dagmar Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81, Košice, Slovakia aut NATIONALLICENCE 700 1- Maďari Aladár Small Animal Clinic, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81, Košice, Slovakia aut NATIONALLICENCE 700 1- Lauková Andrea Institute of Animal Physiology Slovak Academy of Sciences, Šoltésovej 4-6, 040 01, Košice, Slovakia aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/20(2015-10-01), 8681-8690 0175-7598 99:20<8681 2015 99 253