caa a22 4500 605499586 CHVBK 20210128100551.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00253-015-6886-5 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6886-5 Overexpression of yeast thioredoxin TRX2 reduces p53-mediated cell death in yeast [Elektronische Daten] [Yosra Kamoun, Imed Mabrouk, Agnes Delahodde, Fatma Boukid, Ines Yacoubi-Hadj Amor, Raja Mokdad-Gargouri, Ali Gargouri] We have previously shown that overexpression of the human tumor suppressor protein P53 causes cell death of the yeast Saccharomyces cerevisiae. P53 overproduction led to transcriptional downregulation of some yeast genes, such as the TRX1/2 thioredoxin system, which plays a key role in cell protection against various oxidative stresses induced by reactive oxygen species (ROS). In the present work, the impact of TRX2 overexpression on apoptosis mediated by p53 overexpression in yeast is investigated. In yeast cells expressing P53 under an inducible promoter together with TRX2 under a strong constitutive promoter, we showed that Tr2p overproduction reduced the apoptotic effect exerted by P53 and increased the viability of the P53-overproducing cells. Furthermore, measurements of ROS amounts by flow cytometry and fluorescence microscopy indicated that the TRX2 protein acted probably through its increased detoxifying activity on the P53-generated ROS. The steady-state level and activity of P53 were not affected by TRX2 overexpression, as shown by western blotting and functional analysis of separated alleles in yeast (FASAY), respectively. The growth inhibitory effect of P53 was partially reversed by the antioxidant N-acetylcysteine. Our data strengthen the idea that overexpression of a single gene (trx2) decreases the p53-mediated cell death by decreasing ROS accumulation. Springer-Verlag Berlin Heidelberg, 2015 Yeast nationallicence P53 nationallicence trx2 nationallicence Cell death nationallicence ROS nationallicence Kamoun Yosra Laboratory of Molecular Biotechnologie of Eukaryotes, Centre of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia aut Mabrouk Imed Research Unit of Molecular Biology Leukemia and Lymphomas, Faculty of Medicine of Sousse, Sousse,, Tunisia aut Delahodde Agnes Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, CEA, CNRS, Université Paris-Sud, Bat 400, 91405 Orsay Cedex, Orsay,, France aut Boukid Fatma Laboratory of Plant Protection and Improvement, Centre of Biotechnology of Sfax, University of Sfax,, Sfax, Tunisia aut Yacoubi-Hadj Amor Ines Laboratory of Plant Protection and Improvement, Centre of Biotechnology of Sfax, University of Sfax,, Sfax, Tunisia aut Mokdad-Gargouri Raja Laboratory of Molecular Biotechnologie of Eukaryotes, Centre of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia aut Gargouri Ali Laboratory of Molecular Biotechnologie of Eukaryotes, Centre of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/20(2015-10-01), 8619-8628 0175-7598 99:20<8619 2015 99 253 https://doi.org/10.1007/s00253-015-6886-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6886-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kamoun Yosra Laboratory of Molecular Biotechnologie of Eukaryotes, Centre of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia aut NATIONALLICENCE 700 1- Mabrouk Imed Research Unit of Molecular Biology Leukemia and Lymphomas, Faculty of Medicine of Sousse, Sousse,, Tunisia aut NATIONALLICENCE 700 1- Delahodde Agnes Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, CEA, CNRS, Université Paris-Sud, Bat 400, 91405 Orsay Cedex, Orsay,, France aut NATIONALLICENCE 700 1- Boukid Fatma Laboratory of Plant Protection and Improvement, Centre of Biotechnology of Sfax, University of Sfax,, Sfax, Tunisia aut NATIONALLICENCE 700 1- Yacoubi-Hadj Amor Ines Laboratory of Plant Protection and Improvement, Centre of Biotechnology of Sfax, University of Sfax,, Sfax, Tunisia aut NATIONALLICENCE 700 1- Mokdad-Gargouri Raja Laboratory of Molecular Biotechnologie of Eukaryotes, Centre of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia aut NATIONALLICENCE 700 1- Gargouri Ali Laboratory of Molecular Biotechnologie of Eukaryotes, Centre of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/20(2015-10-01), 8619-8628 0175-7598 99:20<8619 2015 99 253