Understanding the intracellular effects of yeast extract on the enhancement of Fc-fusion protein production in Chinese hamster ovary cell culture
| LEADER | caa a22 4500 | ||
|---|---|---|---|
| 001 | 605499594 | ||
| 003 | CHVBK | ||
| 005 | 20210128100551.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 210128e20151001xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-015-6789-5 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6789-5 | ||
| 245 | 0 | 0 | |a Understanding the intracellular effects of yeast extract on the enhancement of Fc-fusion protein production in Chinese hamster ovary cell culture |h [Elektronische Daten] |c [Dongdong Hu, Yating Sun, Xuping Liu, Jintao Liu, Xintao Zhang, Liang Zhao, Haibin Wang, Wen-Song Tan, Li Fan] |
| 520 | 3 | |a Yeast extract (YE), as a non-animal source additive for mammalian cell culture medium, has been widely used for manufacturing of therapeutic proteins. In the present study, one particular YE was found to have significantly improved the specific productivity (q p) of Fc-fusion protein in recombinant Chinese hamster ovary (rCHO) cell culture. In order to elucidate the intracellular effects of YE on protein productivity, steps of the target protein synthesis process were investigated to unveil their variations caused by YE addition. Stepwise analysis on Fc-fusion protein synthesis process showed that YE enhanced Fc-fusion protein gene transcription with cell cycle arrest at G1 phase; mammalian target of rapamycin (mTOR) signaling pathway was activated to enhance the translation of Fc-fusion protein, and the block in post-translational steps of Fc-fusion protein was alleviated by YE addition as well. Our results revealed the responses of multiple protein production steps to the addition of YE and provided a practical guidance for the separation and application of active compounds from hydrolysates. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Chinese hamster ovary cells |2 nationallicence | |
| 690 | 7 | |a Yeast extract |2 nationallicence | |
| 690 | 7 | |a Fc-fusion protein |2 nationallicence | |
| 690 | 7 | |a Specific productivity |2 nationallicence | |
| 690 | 7 | |a Cell cycle arrest |2 nationallicence | |
| 690 | 7 | |a mTOR |2 nationallicence | |
| 700 | 1 | |a Hu |D Dongdong |u State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 309, 200237, Shanghai, People's Republic of China |4 aut | |
| 700 | 1 | |a Sun |D Yating |u State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 309, 200237, Shanghai, People's Republic of China |4 aut | |
| 700 | 1 | |a Liu |D Xuping |u State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 309, 200237, Shanghai, People's Republic of China |4 aut | |
| 700 | 1 | |a Liu |D Jintao |u State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 309, 200237, Shanghai, People's Republic of China |4 aut | |
| 700 | 1 | |a Zhang |D Xintao |u State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 309, 200237, Shanghai, People's Republic of China |4 aut | |
| 700 | 1 | |a Zhao |D Liang |u State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 309, 200237, Shanghai, People's Republic of China |4 aut | |
| 700 | 1 | |a Wang |D Haibin |u Zhejiang Hisun Pharmaceutical Co., Ltd., Fuyang, 311404, Hangzhou, Zhejiang, China |4 aut | |
| 700 | 1 | |a Tan |D Wen-Song |u State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 309, 200237, Shanghai, People's Republic of China |4 aut | |
| 700 | 1 | |a Fan |D Li |u State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 309, 200237, Shanghai, People's Republic of China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/20(2015-10-01), 8429-8440 |x 0175-7598 |q 99:20<8429 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6789-5 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6789-5 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Hu |D Dongdong |u State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 309, 200237, Shanghai, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sun |D Yating |u State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 309, 200237, Shanghai, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Liu |D Xuping |u State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 309, 200237, Shanghai, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Liu |D Jintao |u State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 309, 200237, Shanghai, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhang |D Xintao |u State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 309, 200237, Shanghai, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhao |D Liang |u State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 309, 200237, Shanghai, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wang |D Haibin |u Zhejiang Hisun Pharmaceutical Co., Ltd., Fuyang, 311404, Hangzhou, Zhejiang, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Tan |D Wen-Song |u State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 309, 200237, Shanghai, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Fan |D Li |u State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 309, 200237, Shanghai, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/20(2015-10-01), 8429-8440 |x 0175-7598 |q 99:20<8429 |1 2015 |2 99 |o 253 | ||