caa a22 4500 605499608 CHVBK 20210128100551.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00253-015-6730-y doi (NATIONALLICENCE)springer-10.1007/s00253-015-6730-y Efficient mannitol production by wild-type Lactobacillus reuteri CRL 1101 is attained at constant pH using a simplified culture medium [Elektronische Daten] [Maria Ortiz, Raúl Raya, Fernanda Mozzi] Mannitol is a natural polyol with multiple industrial applications. In this work, mannitol production by Lactobacillus reuteri CRL 1101 was studied at free- and controlled-pH (6.0-4.8) fermentations using a simplified culture medium containing yeast and beef extracts and sugarcane molasses. The activity of mannitol 2-dehydrogenase (MDH), the enzyme responsible for mannitol synthesis, was determined. The effect of the initial biomass concentration was further studied. Mannitol production (41.5±1.1g/l), volumetric productivity (Q Mtl 1.73±0.05g/l h), and yield (Y Mtl 105±11%) were maximum at pH 5.0 after 24h while the highest MDH activity (1.66±0.09U/mg protein) was obtained at pH 6.0. No correlation between mannitol production and MDH activity was observed when varying the culture pH. The increase (up to 2000-fold) in the initial biomass concentration did not improve mannitol formation after 24h although a 2-fold higher amount was produced at 8h using 1 or 2g cell dry weight/l comparing to the control (0.001g cell dry weight/l). Finally, mannitol isolation under optimum fermentation conditions was achieved. The mannitol production obtained in this study is the highest reported so far by a wild-type L. reuteri strain and, more interestingly, using a simplified culture medium. Springer-Verlag Berlin Heidelberg, 2015 Polyol nationallicence Lactic acid bacteria nationallicence Sugarcane molasses nationallicence Fermentation nationallicence Sugar alcohol nationallicence Ortiz Maria Departamento de Tecnología y Desarrollo, Centro de Referencia para Lactobacilos (CERELA)-CONICET, Chacabuco 145, 4000, San Miguel de Tucumán, Argentina aut Raya Raúl Departamento de Tecnología y Desarrollo, Centro de Referencia para Lactobacilos (CERELA)-CONICET, Chacabuco 145, 4000, San Miguel de Tucumán, Argentina aut Mozzi Fernanda Departamento de Tecnología y Desarrollo, Centro de Referencia para Lactobacilos (CERELA)-CONICET, Chacabuco 145, 4000, San Miguel de Tucumán, Argentina aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/20(2015-10-01), 8717-8729 0175-7598 99:20<8717 2015 99 253 https://doi.org/10.1007/s00253-015-6730-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6730-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ortiz Maria Departamento de Tecnología y Desarrollo, Centro de Referencia para Lactobacilos (CERELA)-CONICET, Chacabuco 145, 4000, San Miguel de Tucumán, Argentina aut NATIONALLICENCE 700 1- Raya Raúl Departamento de Tecnología y Desarrollo, Centro de Referencia para Lactobacilos (CERELA)-CONICET, Chacabuco 145, 4000, San Miguel de Tucumán, Argentina aut NATIONALLICENCE 700 1- Mozzi Fernanda Departamento de Tecnología y Desarrollo, Centro de Referencia para Lactobacilos (CERELA)-CONICET, Chacabuco 145, 4000, San Miguel de Tucumán, Argentina aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/20(2015-10-01), 8717-8729 0175-7598 99:20<8717 2015 99 253