Optimization of synergism of a recombinant auxiliary activity 9 from Chaetomium globosum with cellulase in cellulose hydrolysis
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6592-3 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6592-3 | ||
| 245 | 0 | 0 | |a Optimization of synergism of a recombinant auxiliary activity 9 from Chaetomium globosum with cellulase in cellulose hydrolysis |h [Elektronische Daten] |c [In Kim, Ki Nam, Eun Yun, Sooah Kim, Hak Youn, Hee Lee, In-Geol Choi, Kyoung Kim] |
| 520 | 3 | |a Auxiliary activity family 9 (AA9, formerly known as glycoside hydrolase family 61 or polysaccharide monooxygenase) is a group of fungal proteins that were recently found to have a significant synergism with cellulase in cellulose hydrolysis via the oxidative cleavage of glycosidic bonds of cellulose chains. In this study, we report the active expression of a recombinant fungal AA9 from Chaetomium globosum (CgAA9) in a bacterial host, Escherichia coli, and the optimization of its synergistic activity in cellulose hydrolysis by using cellulase. The recombinant CgAA9 (0.9mg/g cellulose) exhibited 1.7-fold synergism in the hydrolysis of Avicel when incubated with 0.9 filter paper units of Celluclast 1.5L/g cellulose. The first study of the active expression of AA9 using a bacterial host and its synergistic optimization could be useful for the industrial application of AA9 for the saccharification of lignocellulose. | |
| 540 | |a The Author(s), 2015 | ||
| 690 | 7 | |a AA9 |2 nationallicence | |
| 690 | 7 | |a GH61 |2 nationallicence | |
| 690 | 7 | |a Synergism |2 nationallicence | |
| 690 | 7 | |a Cellulose hydrolysis |2 nationallicence | |
| 690 | 7 | |a Cellulase |2 nationallicence | |
| 690 | 7 | |a Chaetomium globosum |2 nationallicence | |
| 700 | 1 | |a Kim |D In |u Department of Biotechnology, Korea University Graduate School, 136-713, Seoul, Republic of Korea |4 aut | |
| 700 | 1 | |a Nam |D Ki |u Pohang Accelerator Laboratory, Pohang University of Science and Technology, 790-784, Pohang, Republic of Korea |4 aut | |
| 700 | 1 | |a Yun |D Eun |u Department of Biotechnology, Korea University Graduate School, 136-713, Seoul, Republic of Korea |4 aut | |
| 700 | 1 | |a Kim |D Sooah |u Department of Biotechnology, Korea University Graduate School, 136-713, Seoul, Republic of Korea |4 aut | |
| 700 | 1 | |a Youn |D Hak |u Department of Biotechnology, Korea University Graduate School, 136-713, Seoul, Republic of Korea |4 aut | |
| 700 | 1 | |a Lee |D Hee |u Department of Biotechnology, Korea University Graduate School, 136-713, Seoul, Republic of Korea |4 aut | |
| 700 | 1 | |a Choi |D In-Geol |u Department of Biotechnology, Korea University Graduate School, 136-713, Seoul, Republic of Korea |4 aut | |
| 700 | 1 | |a Kim |D Kyoung |u Department of Biotechnology, Korea University Graduate School, 136-713, Seoul, Republic of Korea |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/20(2015-10-01), 8537-8547 |x 0175-7598 |q 99:20<8537 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6592-3 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6592-3 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kim |D In |u Department of Biotechnology, Korea University Graduate School, 136-713, Seoul, Republic of Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Nam |D Ki |u Pohang Accelerator Laboratory, Pohang University of Science and Technology, 790-784, Pohang, Republic of Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yun |D Eun |u Department of Biotechnology, Korea University Graduate School, 136-713, Seoul, Republic of Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kim |D Sooah |u Department of Biotechnology, Korea University Graduate School, 136-713, Seoul, Republic of Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Youn |D Hak |u Department of Biotechnology, Korea University Graduate School, 136-713, Seoul, Republic of Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lee |D Hee |u Department of Biotechnology, Korea University Graduate School, 136-713, Seoul, Republic of Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Choi |D In-Geol |u Department of Biotechnology, Korea University Graduate School, 136-713, Seoul, Republic of Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kim |D Kyoung |u Department of Biotechnology, Korea University Graduate School, 136-713, Seoul, Republic of Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/20(2015-10-01), 8537-8547 |x 0175-7598 |q 99:20<8537 |1 2015 |2 99 |o 253 | ||