caa a22 4500 605499616 CHVBK 20210128100551.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00253-015-6592-3 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6592-3 Optimization of synergism of a recombinant auxiliary activity 9 from Chaetomium globosum with cellulase in cellulose hydrolysis [Elektronische Daten] [In Kim, Ki Nam, Eun Yun, Sooah Kim, Hak Youn, Hee Lee, In-Geol Choi, Kyoung Kim] Auxiliary activity family 9 (AA9, formerly known as glycoside hydrolase family 61 or polysaccharide monooxygenase) is a group of fungal proteins that were recently found to have a significant synergism with cellulase in cellulose hydrolysis via the oxidative cleavage of glycosidic bonds of cellulose chains. In this study, we report the active expression of a recombinant fungal AA9 from Chaetomium globosum (CgAA9) in a bacterial host, Escherichia coli, and the optimization of its synergistic activity in cellulose hydrolysis by using cellulase. The recombinant CgAA9 (0.9mg/g cellulose) exhibited 1.7-fold synergism in the hydrolysis of Avicel when incubated with 0.9 filter paper units of Celluclast 1.5L/g cellulose. The first study of the active expression of AA9 using a bacterial host and its synergistic optimization could be useful for the industrial application of AA9 for the saccharification of lignocellulose. The Author(s), 2015 AA9 nationallicence GH61 nationallicence Synergism nationallicence Cellulose hydrolysis nationallicence Cellulase nationallicence Chaetomium globosum nationallicence Kim In Department of Biotechnology, Korea University Graduate School, 136-713, Seoul, Republic of Korea aut Nam Ki Pohang Accelerator Laboratory, Pohang University of Science and Technology, 790-784, Pohang, Republic of Korea aut Yun Eun Department of Biotechnology, Korea University Graduate School, 136-713, Seoul, Republic of Korea aut Kim Sooah Department of Biotechnology, Korea University Graduate School, 136-713, Seoul, Republic of Korea aut Youn Hak Department of Biotechnology, Korea University Graduate School, 136-713, Seoul, Republic of Korea aut Lee Hee Department of Biotechnology, Korea University Graduate School, 136-713, Seoul, Republic of Korea aut Choi In-Geol Department of Biotechnology, Korea University Graduate School, 136-713, Seoul, Republic of Korea aut Kim Kyoung Department of Biotechnology, Korea University Graduate School, 136-713, Seoul, Republic of Korea aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/20(2015-10-01), 8537-8547 0175-7598 99:20<8537 2015 99 253 https://doi.org/10.1007/s00253-015-6592-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6592-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kim In Department of Biotechnology, Korea University Graduate School, 136-713, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Nam Ki Pohang Accelerator Laboratory, Pohang University of Science and Technology, 790-784, Pohang, Republic of Korea aut NATIONALLICENCE 700 1- Yun Eun Department of Biotechnology, Korea University Graduate School, 136-713, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Kim Sooah Department of Biotechnology, Korea University Graduate School, 136-713, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Youn Hak Department of Biotechnology, Korea University Graduate School, 136-713, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Lee Hee Department of Biotechnology, Korea University Graduate School, 136-713, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Choi In-Geol Department of Biotechnology, Korea University Graduate School, 136-713, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Kim Kyoung Department of Biotechnology, Korea University Graduate School, 136-713, Seoul, Republic of Korea aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/20(2015-10-01), 8537-8547 0175-7598 99:20<8537 2015 99 253