caa a22 4500 605499640 CHVBK 20210128100552.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00253-015-6860-2 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6860-2 Herboxidiene biosynthesis, production, and structural modifications: prospect for hybrids with related polyketide [Elektronische Daten] [Anaya Pokhrel, Dipesh Dhakal, Amit Jha, Jae Sohng] Herboxidiene is a polyketide with a diverse range of activities, including herbicidal, anti-cholesterol, and pre-mRNA splicing inhibitory effects. Thus, production of the compound on the industrial scale is in high demand, and various rational metabolic engineering approaches have been employed to enhance the yield. Directing the precursors and cofactors pool toward the production of polyketide compounds provides a rationale for developing a good host for polyketide production. Due to multiple promising biological activities, the production of a number of herboxidiene derivatives has been attempted in recent years in a search for the key to improve its potency and to introduce new activities. Structural diversification through combinatorial biosynthesis was attempted, utilizing the heterologous expression of substrate-flexible glucosyltransferase (GT) and cytochrome P450 in Streptomyces chromofuscus to generate structurally and functionally diverse derivatives of herboxidiene. The successful attempt confirmed that the strain was amenable to heterologous expression of foreign polyketide synthase (PKS) or post-PKS modification genes, providing the foundation for generating novel or hybrid polyketides. Springer-Verlag Berlin Heidelberg, 2015 Herboxidiene nationallicence Polyketide nationallicence Enhancement nationallicence Combinatorial biosynthesis nationallicence Hybrid polyketide nationallicence Pokhrel Anaya Department of BT-Convergent Pharmaceutical Engineering, Institute of Biomolecule Reconstruction (iBR), Sun Moon University, 70, Sunmoon-Ro 221, Tangjeong-myeon, 333-708, Asan, Chungnam, Republic of Korea aut Dhakal Dipesh Department of BT-Convergent Pharmaceutical Engineering, Institute of Biomolecule Reconstruction (iBR), Sun Moon University, 70, Sunmoon-Ro 221, Tangjeong-myeon, 333-708, Asan, Chungnam, Republic of Korea aut Jha Amit Department of BT-Convergent Pharmaceutical Engineering, Institute of Biomolecule Reconstruction (iBR), Sun Moon University, 70, Sunmoon-Ro 221, Tangjeong-myeon, 333-708, Asan, Chungnam, Republic of Korea aut Sohng Jae Department of BT-Convergent Pharmaceutical Engineering, Institute of Biomolecule Reconstruction (iBR), Sun Moon University, 70, Sunmoon-Ro 221, Tangjeong-myeon, 333-708, Asan, Chungnam, Republic of Korea aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/20(2015-10-01), 8351-8362 0175-7598 99:20<8351 2015 99 253 https://doi.org/10.1007/s00253-015-6860-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6860-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pokhrel Anaya Department of BT-Convergent Pharmaceutical Engineering, Institute of Biomolecule Reconstruction (iBR), Sun Moon University, 70, Sunmoon-Ro 221, Tangjeong-myeon, 333-708, Asan, Chungnam, Republic of Korea aut NATIONALLICENCE 700 1- Dhakal Dipesh Department of BT-Convergent Pharmaceutical Engineering, Institute of Biomolecule Reconstruction (iBR), Sun Moon University, 70, Sunmoon-Ro 221, Tangjeong-myeon, 333-708, Asan, Chungnam, Republic of Korea aut NATIONALLICENCE 700 1- Jha Amit Department of BT-Convergent Pharmaceutical Engineering, Institute of Biomolecule Reconstruction (iBR), Sun Moon University, 70, Sunmoon-Ro 221, Tangjeong-myeon, 333-708, Asan, Chungnam, Republic of Korea aut NATIONALLICENCE 700 1- Sohng Jae Department of BT-Convergent Pharmaceutical Engineering, Institute of Biomolecule Reconstruction (iBR), Sun Moon University, 70, Sunmoon-Ro 221, Tangjeong-myeon, 333-708, Asan, Chungnam, Republic of Korea aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/20(2015-10-01), 8351-8362 0175-7598 99:20<8351 2015 99 253