caa a22 4500 605499675 CHVBK 20210128100552.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00253-015-6731-x doi (NATIONALLICENCE)springer-10.1007/s00253-015-6731-x Impact of the antifungal protein PgAFP from Penicillium chrysogenum on the protein profile in Aspergillus flavus [Elektronische Daten] [Josué Delgado, Rebecca Owens, Sean Doyle, Miguel Asensio, Félix Núñez] Antifungal proteins produced by molds are generally small, highly basic, and cysteine-rich. The best known effects of these proteins include morphological changes, metabolic inactivation, and membrane perturbation on sensitive fungi. Reactive oxygen species (ROS) generation leads to apoptosis, with G protein playing a key role in transduction of cell death signals. The antifungal protein PgAFP from Penicillium chrysogenum inhibits growth of some toxigenic molds. Here we analyzed the effect of the antifungal protein PgAFP on the growth of Aspergillus flavus. For this, comparative proteomic analysis was used to identify the whole protein profile and protein change in abundance after PgAFP treatment. PgAFP provoked metabolic changes related to reduced energy metabolism, cell wall integrity alteration, and increased stress response due to higher levels of ROS. The observed changes in protein abundance, favoring a higher glutathione concentration as well as the increased abundance in heat shock proteins, do not seem to be enough to avoid necrosis. The decreased chitin deposition observed in PgAFP-treated A. flavus is attributed to a lower relative quantity of Rho1. The reduced relative abundance of a β subunit of G protein seems to be the underlying reason for modulation of apoptosis in PgAFP-treated A. flavus hyphae. We propose Rho1 and G protein subunit β CpcB to be the main factors in the mode of action of PgAFP in A. flavus. Additionally, enzymes essential for the biosynthesis of aflatoxin were no longer detectable in A. flavus hyphae at 24h, following treatment with PgAFP. This presents a promising effect of PgAFP, which may prevent A. flavus from producing mycotoxins. However, the impact of PgAFP on actual aflatoxin production requires further study. Springer-Verlag Berlin Heidelberg, 2015 Antifungal compounds nationallicence Toxigenic fungi nationallicence Aspergillus flavus nationallicence Apoptosis nationallicence Proteomics nationallicence Delgado Josué Food Hygiene and Safety, Institute of Meat Products, University of Extremadura, Cáceres, Spain aut Owens Rebecca Department of Biology, Maynooth University, Maynooth, Co. Kildare, Ireland aut Doyle Sean Department of Biology, Maynooth University, Maynooth, Co. Kildare, Ireland aut Asensio Miguel Food Hygiene and Safety, Institute of Meat Products, University of Extremadura, Cáceres, Spain aut Núñez Félix Food Hygiene and Safety, Institute of Meat Products, University of Extremadura, Cáceres, Spain aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/20(2015-10-01), 8701-8715 0175-7598 99:20<8701 2015 99 253 https://doi.org/10.1007/s00253-015-6731-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6731-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Delgado Josué Food Hygiene and Safety, Institute of Meat Products, University of Extremadura, Cáceres, Spain aut NATIONALLICENCE 700 1- Owens Rebecca Department of Biology, Maynooth University, Maynooth, Co. Kildare, Ireland aut NATIONALLICENCE 700 1- Doyle Sean Department of Biology, Maynooth University, Maynooth, Co. Kildare, Ireland aut NATIONALLICENCE 700 1- Asensio Miguel Food Hygiene and Safety, Institute of Meat Products, University of Extremadura, Cáceres, Spain aut NATIONALLICENCE 700 1- Núñez Félix Food Hygiene and Safety, Institute of Meat Products, University of Extremadura, Cáceres, Spain aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/20(2015-10-01), 8701-8715 0175-7598 99:20<8701 2015 99 253