Identification of sucrose synthase in nonphotosynthetic bacteria and characterization of the recombinant enzymes
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| 001 | 605499691 | ||
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| 005 | 20210128100552.0 | ||
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| 008 | 210128e20151001xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-015-6548-7 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6548-7 | ||
| 245 | 0 | 0 | |a Identification of sucrose synthase in nonphotosynthetic bacteria and characterization of the recombinant enzymes |h [Elektronische Daten] |c [Margo Diricks, Frederik De Bruyn, Paul Van Daele, Maarten Walmagh, Tom Desmet] |
| 520 | 3 | |a Sucrose synthase (SuSy) catalyzes the reversible conversion of sucrose and a nucleoside diphosphate into fructose and nucleotide (NDP)-glucose. To date, only SuSy's from plants and cyanobacteria, both photosynthetic organisms, have been characterized. Here, four prokaryotic SuSy enzymes from the nonphotosynthetic organisms Nitrosomonas Europaea (SuSyNe), Acidithiobacillus caldus (SuSyAc), Denitrovibrio acetiphilus (SusyDa), and Melioribacter roseus (SuSyMr) were recombinantly expressed in Escherichia coli and thoroughly characterized. The purified enzymes were found to display high-temperature optima (up to 80 °C), high activities (up to 125 U/mg), and high thermostability (up to 15 min at 60 °C). Furthermore, SuSyAc, SuSyNe, and SuSyDa showed a clear preference for ADP as nucleotide, as opposed to plant SuSy's which prefer UDP. A structural and mutational analysis was performed to elucidate the difference in NDP preference between eukaryotic and prokaryotic SuSy's. Finally, the physiological relevance of this enzyme specificity is discussed in the context of metabolic pathways and genomic organization. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Sucrose synthase |2 nationallicence | |
| 690 | 7 | |a Sucrose metabolism |2 nationallicence | |
| 690 | 7 | |a Photosynthesis |2 nationallicence | |
| 690 | 7 | |a Nucleotide sugar |2 nationallicence | |
| 700 | 1 | |a Diricks |D Margo |u Centre for Industrial Biotechnology and Biocatalysis, Department of Biochemical and Microbial Technology, Ghent University, Coupure Links 653, 9000, Ghent, Belgium |4 aut | |
| 700 | 1 | |a De Bruyn |D Frederik |u Centre for Industrial Biotechnology and Biocatalysis, Department of Biochemical and Microbial Technology, Ghent University, Coupure Links 653, 9000, Ghent, Belgium |4 aut | |
| 700 | 1 | |a Van Daele |D Paul |u Centre for Industrial Biotechnology and Biocatalysis, Department of Biochemical and Microbial Technology, Ghent University, Coupure Links 653, 9000, Ghent, Belgium |4 aut | |
| 700 | 1 | |a Walmagh |D Maarten |u Centre for Industrial Biotechnology and Biocatalysis, Department of Biochemical and Microbial Technology, Ghent University, Coupure Links 653, 9000, Ghent, Belgium |4 aut | |
| 700 | 1 | |a Desmet |D Tom |u Centre for Industrial Biotechnology and Biocatalysis, Department of Biochemical and Microbial Technology, Ghent University, Coupure Links 653, 9000, Ghent, Belgium |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/20(2015-10-01), 8465-8474 |x 0175-7598 |q 99:20<8465 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6548-7 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6548-7 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Diricks |D Margo |u Centre for Industrial Biotechnology and Biocatalysis, Department of Biochemical and Microbial Technology, Ghent University, Coupure Links 653, 9000, Ghent, Belgium |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a De Bruyn |D Frederik |u Centre for Industrial Biotechnology and Biocatalysis, Department of Biochemical and Microbial Technology, Ghent University, Coupure Links 653, 9000, Ghent, Belgium |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Van Daele |D Paul |u Centre for Industrial Biotechnology and Biocatalysis, Department of Biochemical and Microbial Technology, Ghent University, Coupure Links 653, 9000, Ghent, Belgium |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Walmagh |D Maarten |u Centre for Industrial Biotechnology and Biocatalysis, Department of Biochemical and Microbial Technology, Ghent University, Coupure Links 653, 9000, Ghent, Belgium |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Desmet |D Tom |u Centre for Industrial Biotechnology and Biocatalysis, Department of Biochemical and Microbial Technology, Ghent University, Coupure Links 653, 9000, Ghent, Belgium |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/20(2015-10-01), 8465-8474 |x 0175-7598 |q 99:20<8465 |1 2015 |2 99 |o 253 | ||