The role of autophagy in the cytotoxicity induced by recombinant human arginase in laryngeal squamous cell carcinoma
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| 008 | 210128e20151001xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-015-6565-6 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6565-6 | ||
| 245 | 0 | 4 | |a The role of autophagy in the cytotoxicity induced by recombinant human arginase in laryngeal squamous cell carcinoma |h [Elektronische Daten] |c [Chen Lin, Ziyu Wang, Li Li, Yong He, Jiajun Fan, Zhongyu Liu, Shuwei Zhao, Dianwen Ju] |
| 520 | 3 | |a Laryngeal squamous cell carcinoma (LSCC), one of the most common malignant solid tumors in the world, has a high rate of mortality, recurrence, and metastasis. Recombinant human arginase (rhArg) recently has been developed in arginine deprivation therapy for a number of malignant tumors. In the present study, we observed that rhArg triggered significant cytotoxicity in human laryngeal squamous cell carcinoma Tu212 cells. Meanwhile, we observed that rhArg simultaneously activated autophagic flux in Tu212 cells, which was demonstrated by the accumulation of autophagosome and light chain 3 II (LC3-II). And, we explored the role of autophagy in cytotoxicity induced by rhArg in Tu212 cells. Autophagy inhibitors including chloroquine (CQ) and bafilomycin A1 (Baf A1) enhanced cytotoxicity induced by rhArg, implying the protective role of autophagy in rhArg-treated Tu212 cells. Moreover, Akt/mTOR signaling pathway was most possibly to participate in the rhArg-induced autophagy. Meanwhile, rhArg could upregulate the phosphorylation of ERK1/2 in a time-dependent manner. Therefore, all the results illuminated the cytoprotective role of autophagy in the treatment of rhArg in laryngeal squamous carcinoma Tu212 cells and provided a potential approach for LSCC therapy by rhArg combined with autophagy inhibitors. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Autophagy |2 nationallicence | |
| 690 | 7 | |a Recombinant human arginase |2 nationallicence | |
| 690 | 7 | |a Laryngeal squamous cell carcinoma |2 nationallicence | |
| 690 | 7 | |a Cytotoxicity |2 nationallicence | |
| 700 | 1 | |a Lin |D Chen |u Department of Otolaryngology, Changzheng Hospital, Second Military Medical University, 200003, Shanghai, China |4 aut | |
| 700 | 1 | |a Wang |D Ziyu |u Department of Biosynthesis & Key Lab of Smart Drug Delivery of Ministry of Education, School of Pharmacy, Fudan University, Shanghai, China |4 aut | |
| 700 | 1 | |a Li |D Li |u Department of Otolaryngology, Changzheng Hospital, Second Military Medical University, 200003, Shanghai, China |4 aut | |
| 700 | 1 | |a He |D Yong |u Department of Otolaryngology, The Affiliated Hospital of School of Medicine of Ningbo University, Ningbo, Zhejiang Province, China |4 aut | |
| 700 | 1 | |a Fan |D Jiajun |u Department of Biosynthesis & Key Lab of Smart Drug Delivery of Ministry of Education, School of Pharmacy, Fudan University, Shanghai, China |4 aut | |
| 700 | 1 | |a Liu |D Zhongyu |u Department of Otolaryngology, Changzheng Hospital, Second Military Medical University, 200003, Shanghai, China |4 aut | |
| 700 | 1 | |a Zhao |D Shuwei |u Department of Otolaryngology, Changzheng Hospital, Second Military Medical University, 200003, Shanghai, China |4 aut | |
| 700 | 1 | |a Ju |D Dianwen |u Department of Biosynthesis & Key Lab of Smart Drug Delivery of Ministry of Education, School of Pharmacy, Fudan University, Shanghai, China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/20(2015-10-01), 8487-8494 |x 0175-7598 |q 99:20<8487 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6565-6 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6565-6 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lin |D Chen |u Department of Otolaryngology, Changzheng Hospital, Second Military Medical University, 200003, Shanghai, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wang |D Ziyu |u Department of Biosynthesis & Key Lab of Smart Drug Delivery of Ministry of Education, School of Pharmacy, Fudan University, Shanghai, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Li |D Li |u Department of Otolaryngology, Changzheng Hospital, Second Military Medical University, 200003, Shanghai, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a He |D Yong |u Department of Otolaryngology, The Affiliated Hospital of School of Medicine of Ningbo University, Ningbo, Zhejiang Province, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Fan |D Jiajun |u Department of Biosynthesis & Key Lab of Smart Drug Delivery of Ministry of Education, School of Pharmacy, Fudan University, Shanghai, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Liu |D Zhongyu |u Department of Otolaryngology, Changzheng Hospital, Second Military Medical University, 200003, Shanghai, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhao |D Shuwei |u Department of Otolaryngology, Changzheng Hospital, Second Military Medical University, 200003, Shanghai, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ju |D Dianwen |u Department of Biosynthesis & Key Lab of Smart Drug Delivery of Ministry of Education, School of Pharmacy, Fudan University, Shanghai, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/20(2015-10-01), 8487-8494 |x 0175-7598 |q 99:20<8487 |1 2015 |2 99 |o 253 | ||