caa a22 4500 605499748 CHVBK 20210128100552.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00253-015-6750-7 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6750-7 Ammonia-oxidizing archaea have better adaptability in oxygenated/hypoxic alternant conditions compared to ammonia-oxidizing bacteria [Elektronische Daten] [Shuai Liu, Baolan Hu, Zhanfei He, Bin Zhang, Guangming Tian, Ping Zheng, Fang Fang] Ammonia oxidation is performed by both ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB). Few studies compared the adaptability of AOA and AOB for oxygenated/hypoxic alternant conditions in water-level-fluctuating zones. Here, using qPCR and 454 high-throughput sequencing of functional amoA genes of AOA and AOB, we examined the changes of abundances, diversities, and community structures of AOA and AOB in periodically flooded soils compared to the non-flooded soils in Three Gorges Reservoir. The increased AOA operational taxonomic unit (OTU) numbers and the higher ratios of abundance (AOA:AOB) in the periodically flooded soils suggested AOA have better adaptability for oxygenated/hypoxic alternant conditions in the water-level-fluctuating zones in the Three Gorges Reservoir and probably responsible for the ammonia oxidation there. Canonical correspondence analysis (CCA) showed that oxidation-reduction potential (ORP) had the most significant effect on the community distribution of AOA (p<0.01). Pearson analysis also indicated that ORP was the most important factor influencing the abundances and diversities of ammonia-oxidizing microbes. ORP was significantly negatively correlated with AOA OTU numbers (p<0.05), ratio of OTU numbers (AOA:AOB) (p<0.01), and ratio of amoA gene abundances (AOA:AOB) (p<0.05). ORP was also significantly positively correlated with AOB abundance (p<0.05). Springer-Verlag Berlin Heidelberg, 2015 Ammonia-oxidizing archaea (AOA) nationallicence Ammonia-oxidizing bacteria (AOB) nationallicence Water-level-fluctuating zones nationallicence Three Gorges Reservoir nationallicence Oxygenated/hypoxic alternant conditions nationallicence Liu Shuai Department of Environmental Engineering, Zhejiang University, Hangzhou, China aut Hu Baolan Department of Environmental Engineering, Zhejiang University, Hangzhou, China aut He Zhanfei Department of Environmental Engineering, Zhejiang University, Hangzhou, China aut Zhang Bin Faculty of Urban Construction and Environmental Engineering, Chongqing University, Chongqing, China aut Tian Guangming Department of Environmental Engineering, Zhejiang University, Hangzhou, China aut Zheng Ping Department of Environmental Engineering, Zhejiang University, Hangzhou, China aut Fang Fang Faculty of Urban Construction and Environmental Engineering, Chongqing University, Chongqing, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/20(2015-10-01), 8587-8596 0175-7598 99:20<8587 2015 99 253 https://doi.org/10.1007/s00253-015-6750-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6750-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Shuai Department of Environmental Engineering, Zhejiang University, Hangzhou, China aut NATIONALLICENCE 700 1- Hu Baolan Department of Environmental Engineering, Zhejiang University, Hangzhou, China aut NATIONALLICENCE 700 1- He Zhanfei Department of Environmental Engineering, Zhejiang University, Hangzhou, China aut NATIONALLICENCE 700 1- Zhang Bin Faculty of Urban Construction and Environmental Engineering, Chongqing University, Chongqing, China aut NATIONALLICENCE 700 1- Tian Guangming Department of Environmental Engineering, Zhejiang University, Hangzhou, China aut NATIONALLICENCE 700 1- Zheng Ping Department of Environmental Engineering, Zhejiang University, Hangzhou, China aut NATIONALLICENCE 700 1- Fang Fang Faculty of Urban Construction and Environmental Engineering, Chongqing University, Chongqing, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/20(2015-10-01), 8587-8596 0175-7598 99:20<8587 2015 99 253