caa a22 4500 605499772 CHVBK 20210128100552.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00253-014-6201-x doi (NATIONALLICENCE)springer-10.1007/s00253-014-6201-x α- N -Acetylglucosaminidase from Bifidobacterium bifidum specifically hydrolyzes α-linked N -acetylglucosamine at nonreducing terminus of O -glycan on gastric mucin [Elektronische Daten] [Yoshimi Shimada, Yuka Watanabe, Takura Wakinaka, Yoshihisa Funeno, Masayuki Kubota, Thida Chaiwangsri, Shin Kurihara, Kenji Yamamoto, Takane Katayama, Hisashi Ashida] α-Linked N-acetylglucosamine is one of the major glyco-epitopes in O-glycan of gastroduodenal mucin. Here, we identified glycoside hydrolase (GH) family 89 α-N-acetylglucosaminidase, termed AgnB, from Bifidobacterium bifidum JCM 1254, which is essentially specific to GlcNAcα1-4Gal structure. AgnB is a membrane-anchored extracellular enzyme consisting of a GH89 domain and four carbohydrate-binding module (CBM) 32 domains. Among four CBM32 domains, three tandem ones at C-terminus showed to bind porcine gastric mucin, suggesting that these domains enhance the enzyme activity by increasing affinity for multivalent substrates. AgnB might be important for assimilation of gastroduodenal mucin by B. bifidum and also applicable to production of prebiotic oligosaccharides from porcine gastric mucin. Springer-Verlag Berlin Heidelberg, 2014 α- N -Acetylglucosaminidase nationallicence Bifidobacterium bifidum nationallicence CBM32 nationallicence GH89 nationallicence Mucin nationallicence Probiotics nationallicence Shimada Yoshimi Faculty of Biology-Oriented Science and Technology, Kinki University, 649-6493, Kinokawa, Wakayama, Japan aut Watanabe Yuka Graduate School of Biostudies, Kyoto University, Sakyo-ku, 606-8502, Kyoto, Japan aut Wakinaka Takura Graduate School of Biostudies, Kyoto University, Sakyo-ku, 606-8502, Kyoto, Japan aut Funeno Yoshihisa Faculty of Biology-Oriented Science and Technology, Kinki University, 649-6493, Kinokawa, Wakayama, Japan aut Kubota Masayuki Faculty of Biology-Oriented Science and Technology, Kinki University, 649-6493, Kinokawa, Wakayama, Japan aut Chaiwangsri Thida School of Medical Science, University of Phayao, 56000, Phayao, Thailand aut Kurihara Shin Host-Microbe Interaction Research Laboratory, Ishikawa Prefectural University, 921-8836, Nonoichi, Ishikawa, Japan aut Yamamoto Kenji Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, 921-8836, Nonoichi, Ishikawa, Japan aut Katayama Takane Host-Microbe Interaction Research Laboratory, Ishikawa Prefectural University, 921-8836, Nonoichi, Ishikawa, Japan aut Ashida Hisashi Faculty of Biology-Oriented Science and Technology, Kinki University, 649-6493, Kinokawa, Wakayama, Japan aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/9(2015-05-01), 3941-3948 0175-7598 99:9<3941 2015 99 253 https://doi.org/10.1007/s00253-014-6201-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6201-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shimada Yoshimi Faculty of Biology-Oriented Science and Technology, Kinki University, 649-6493, Kinokawa, Wakayama, Japan aut NATIONALLICENCE 700 1- Watanabe Yuka Graduate School of Biostudies, Kyoto University, Sakyo-ku, 606-8502, Kyoto, Japan aut NATIONALLICENCE 700 1- Wakinaka Takura Graduate School of Biostudies, Kyoto University, Sakyo-ku, 606-8502, Kyoto, Japan aut NATIONALLICENCE 700 1- Funeno Yoshihisa Faculty of Biology-Oriented Science and Technology, Kinki University, 649-6493, Kinokawa, Wakayama, Japan aut NATIONALLICENCE 700 1- Kubota Masayuki Faculty of Biology-Oriented Science and Technology, Kinki University, 649-6493, Kinokawa, Wakayama, Japan aut NATIONALLICENCE 700 1- Chaiwangsri Thida School of Medical Science, University of Phayao, 56000, Phayao, Thailand aut NATIONALLICENCE 700 1- Kurihara Shin Host-Microbe Interaction Research Laboratory, Ishikawa Prefectural University, 921-8836, Nonoichi, Ishikawa, Japan aut NATIONALLICENCE 700 1- Yamamoto Kenji Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, 921-8836, Nonoichi, Ishikawa, Japan aut NATIONALLICENCE 700 1- Katayama Takane Host-Microbe Interaction Research Laboratory, Ishikawa Prefectural University, 921-8836, Nonoichi, Ishikawa, Japan aut NATIONALLICENCE 700 1- Ashida Hisashi Faculty of Biology-Oriented Science and Technology, Kinki University, 649-6493, Kinokawa, Wakayama, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/9(2015-05-01), 3941-3948 0175-7598 99:9<3941 2015 99 253