Identification and quantification of Thiothrix eikelboomii using qPCR for early detection of bulking incidents in a full-scale water reclamation plant
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6230-5 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6230-5 | ||
| 245 | 0 | 0 | |a Identification and quantification of Thiothrix eikelboomii using qPCR for early detection of bulking incidents in a full-scale water reclamation plant |h [Elektronische Daten] |c [Pitiporn Asvapathanagul, Betty Olson, Phillip Gedalanga, Ardalan Hashemi, Zhonghua Huang, Jason La] |
| 520 | 3 | |a This study found that the ratio of Thiothrix eikelboomii to total bacterial concentrations (TH/TB) (%) was a better indicator of bulking incidents affecting effluent quality compared to absolute T. eikelboomii abundance alone. This was determined using a genus-specific Thiothrix quantitative PCR primer and probe set, which was developed in this study to monitor specific Thiothrix populations over a 1-year period. T. eikelboomii was identified as the source of bulking incidents based on sequencing of the 16S rRNA gene at a nitrifying-denitrifying wastewater treatment plant. Peak T. eikelboomii concentrations observed in March, April, and July 2009 were 2.32 × 1010, 2.64 × 1010, and 1.84 × 1010 cells/l, respectively. The highest fraction of T. eikelboomii to total bacterial population was measured at 0.24% in March, and a ratio >0.19% caused increases of suspended solids and biochemical oxygen demand in the secondary effluent. Additionally, food/mass ratios, dissolved oxygen concentrations in the anoxic selector, and ammonium ion concentrations in the primary effluent were three parameters displaying statistically significant correlations (r = 0.40, r = 0.50, and r = 0.32, respectively) to Thiothrix spp. abundance in an aeration tank. No bulking events caused by T. eikelboomii occurred when the dissolved oxygen concentrations in the anoxic selector was maintained at lower than 0.12mg/l and the TH/TB ratios were <0.10%. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Thiothrix eikelboomii |2 nationallicence | |
| 690 | 7 | |a Type 021N |2 nationallicence | |
| 690 | 7 | |a Bulking |2 nationallicence | |
| 690 | 7 | |a DO in anoxic selector |2 nationallicence | |
| 690 | 7 | |a Activated sludge |2 nationallicence | |
| 690 | 7 | |a Ratio Thiothrix eikelboomii to total bacteria (TH/TB) |2 nationallicence | |
| 700 | 1 | |a Asvapathanagul |D Pitiporn |u Department of Civil Engineering and Construction Engineering Management, California State University, Long Beach, 90840, Long Beach, CA, USA |4 aut | |
| 700 | 1 | |a Olson |D Betty |u Department of Civil and Environmental Engineering, University of California, Irvine, 92697, Irvine, CA, USA |4 aut | |
| 700 | 1 | |a Gedalanga |D Phillip |u Department of Civil and Environmental Engineering, University of California, Los Angeles, 90095, Los Angeles, CA, USA |4 aut | |
| 700 | 1 | |a Hashemi |D Ardalan |u Civil and Environmental Engineering, University of California, Los Angeles, USA |4 aut | |
| 700 | 1 | |a Huang |D Zhonghua |u Department of Environment and Engineering, Nanjing University of Science and Technology, 210094, Nanjing, China |4 aut | |
| 700 | 1 | |a La |D Jason |u Irvine Ranch Water District, 92612, Irvine, CA, USA |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/9(2015-05-01), 4045-4057 |x 0175-7598 |q 99:9<4045 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6230-5 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6230-5 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Asvapathanagul |D Pitiporn |u Department of Civil Engineering and Construction Engineering Management, California State University, Long Beach, 90840, Long Beach, CA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Olson |D Betty |u Department of Civil and Environmental Engineering, University of California, Irvine, 92697, Irvine, CA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Gedalanga |D Phillip |u Department of Civil and Environmental Engineering, University of California, Los Angeles, 90095, Los Angeles, CA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Hashemi |D Ardalan |u Civil and Environmental Engineering, University of California, Los Angeles, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Huang |D Zhonghua |u Department of Environment and Engineering, Nanjing University of Science and Technology, 210094, Nanjing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a La |D Jason |u Irvine Ranch Water District, 92612, Irvine, CA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/9(2015-05-01), 4045-4057 |x 0175-7598 |q 99:9<4045 |1 2015 |2 99 |o 253 | ||