caa a22 4500 605499861 CHVBK 20210128100553.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00253-014-6230-5 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6230-5 Identification and quantification of Thiothrix eikelboomii using qPCR for early detection of bulking incidents in a full-scale water reclamation plant [Elektronische Daten] [Pitiporn Asvapathanagul, Betty Olson, Phillip Gedalanga, Ardalan Hashemi, Zhonghua Huang, Jason La] This study found that the ratio of Thiothrix eikelboomii to total bacterial concentrations (TH/TB) (%) was a better indicator of bulking incidents affecting effluent quality compared to absolute T. eikelboomii abundance alone. This was determined using a genus-specific Thiothrix quantitative PCR primer and probe set, which was developed in this study to monitor specific Thiothrix populations over a 1-year period. T. eikelboomii was identified as the source of bulking incidents based on sequencing of the 16S rRNA gene at a nitrifying-denitrifying wastewater treatment plant. Peak T. eikelboomii concentrations observed in March, April, and July 2009 were 2.32 × 1010, 2.64 × 1010, and 1.84 × 1010 cells/l, respectively. The highest fraction of T. eikelboomii to total bacterial population was measured at 0.24% in March, and a ratio >0.19% caused increases of suspended solids and biochemical oxygen demand in the secondary effluent. Additionally, food/mass ratios, dissolved oxygen concentrations in the anoxic selector, and ammonium ion concentrations in the primary effluent were three parameters displaying statistically significant correlations (r = 0.40, r = 0.50, and r = 0.32, respectively) to Thiothrix spp. abundance in an aeration tank. No bulking events caused by T. eikelboomii occurred when the dissolved oxygen concentrations in the anoxic selector was maintained at lower than 0.12mg/l and the TH/TB ratios were <0.10%. Springer-Verlag Berlin Heidelberg, 2015 Thiothrix eikelboomii nationallicence Type 021N nationallicence Bulking nationallicence DO in anoxic selector nationallicence Activated sludge nationallicence Ratio Thiothrix eikelboomii to total bacteria (TH/TB) nationallicence Asvapathanagul Pitiporn Department of Civil Engineering and Construction Engineering Management, California State University, Long Beach, 90840, Long Beach, CA, USA aut Olson Betty Department of Civil and Environmental Engineering, University of California, Irvine, 92697, Irvine, CA, USA aut Gedalanga Phillip Department of Civil and Environmental Engineering, University of California, Los Angeles, 90095, Los Angeles, CA, USA aut Hashemi Ardalan Civil and Environmental Engineering, University of California, Los Angeles, USA aut Huang Zhonghua Department of Environment and Engineering, Nanjing University of Science and Technology, 210094, Nanjing, China aut La Jason Irvine Ranch Water District, 92612, Irvine, CA, USA aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/9(2015-05-01), 4045-4057 0175-7598 99:9<4045 2015 99 253 https://doi.org/10.1007/s00253-014-6230-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6230-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Asvapathanagul Pitiporn Department of Civil Engineering and Construction Engineering Management, California State University, Long Beach, 90840, Long Beach, CA, USA aut NATIONALLICENCE 700 1- Olson Betty Department of Civil and Environmental Engineering, University of California, Irvine, 92697, Irvine, CA, USA aut NATIONALLICENCE 700 1- Gedalanga Phillip Department of Civil and Environmental Engineering, University of California, Los Angeles, 90095, Los Angeles, CA, USA aut NATIONALLICENCE 700 1- Hashemi Ardalan Civil and Environmental Engineering, University of California, Los Angeles, USA aut NATIONALLICENCE 700 1- Huang Zhonghua Department of Environment and Engineering, Nanjing University of Science and Technology, 210094, Nanjing, China aut NATIONALLICENCE 700 1- La Jason Irvine Ranch Water District, 92612, Irvine, CA, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/9(2015-05-01), 4045-4057 0175-7598 99:9<4045 2015 99 253