caa a22 4500 60549987X CHVBK 20210128100553.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00253-015-6475-7 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6475-7 Mathematical modeling of the fermentation of acid-hydrolyzed pyrolytic sugars to ethanol by the engineered strain Escherichia coli ACCC 11177 [Elektronische Daten] [Dongdong Chang, Zhisheng Yu, Zia Islam, Hongxun Zhang] Pyrolysate from waste cotton was acid hydrolyzed and detoxified to yield pyrolytic sugars, which were fermented to ethanol by the strain Escherichia coli ACCC 11177. Mathematical models based on the fermentation data were also constructed. Pyrolysate containing an initial levoglucosan concentration of 146.34g/L gave a glucose yield of 150% after hydrolysis, suggesting that other compounds were hydrolyzed to glucose as well. Ethyl acetate-based extraction of bacterial growth inhibitors with an ethyl acetate/hydrolysate ratio of 1:0.5 enabled hydrolysate fermentation by E. coli ACCC 11177, without a standard absorption treatment. Batch processing in a fermenter exhibited a maximum ethanol yield and productivity of 0.41g/g and 0.93g/L·h−1, respectively. The cell growth rate (r x ) was consistent with a logistic equation r x = 0.21 1 − X 3.75 X $$ {r}_x=0.21\left(1-\frac{X}{3.75}\right)X $$ , which was determined as a function of cell growth (X). Glucose consumption rate (r s ) and ethanol formation rate (r p ) were accurately validated by the equations r s = 0.25 d X d t + 0.47 X $$ {r}_s=0.25\frac{dX}{dt}+0.47X $$ and r p = 0.05 d X d t + 0.29 X $$ {r}_p=0.05\frac{dX}{dt}+0.29X $$ , respectively. Together, our results suggest that combining mathematical models with fermenter fermentation processes can enable optimized ethanol production from cellulosic pyrolysate with E. coli. Similar approaches may facilitate the production of other commercially important organic substances. Springer-Verlag Berlin Heidelberg, 2015 Levoglucosan nationallicence Hydrolysis nationallicence E. coli nationallicence Ethanol nationallicence Mathematical model nationallicence Chang Dongdong College of Resources and Environment, University of Chinese Academy of Sciences, 19 A Yuquan Road, Shijingshan District, 100049, Beijing, People's Republic of China aut Yu Zhisheng College of Resources and Environment, University of Chinese Academy of Sciences, 19 A Yuquan Road, Shijingshan District, 100049, Beijing, People's Republic of China aut Islam Zia College of Resources and Environment, University of Chinese Academy of Sciences, 19 A Yuquan Road, Shijingshan District, 100049, Beijing, People's Republic of China aut Zhang Hongxun College of Resources and Environment, University of Chinese Academy of Sciences, 19 A Yuquan Road, Shijingshan District, 100049, Beijing, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/9(2015-05-01), 4093-4105 0175-7598 99:9<4093 2015 99 253 https://doi.org/10.1007/s00253-015-6475-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6475-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chang Dongdong College of Resources and Environment, University of Chinese Academy of Sciences, 19 A Yuquan Road, Shijingshan District, 100049, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Yu Zhisheng College of Resources and Environment, University of Chinese Academy of Sciences, 19 A Yuquan Road, Shijingshan District, 100049, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Islam Zia College of Resources and Environment, University of Chinese Academy of Sciences, 19 A Yuquan Road, Shijingshan District, 100049, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Hongxun College of Resources and Environment, University of Chinese Academy of Sciences, 19 A Yuquan Road, Shijingshan District, 100049, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/9(2015-05-01), 4093-4105 0175-7598 99:9<4093 2015 99 253