Response of denitrifying genes coding for nitrite ( nirK or nirS ) and nitrous oxide ( nosZ ) reductases to different physico-chemical parameters during agricultural waste composting

Verfasser / Beitragende:
[Lihua Zhang, Guangming Zeng, Jiachao Zhang, Yaoning Chen, Man Yu, Lunhui Lu, Hui Li, Yuan Zhu, Yujie Yuan, Aizhi Huang, Ling He]
Ort, Verlag, Jahr:
2015
Enthalten in:
Applied Microbiology and Biotechnology, 99/9(2015-05-01), 4059-4070
Format:
Artikel (online)
ID: 605499934
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024 7 0 |a 10.1007/s00253-014-6293-3  |2 doi 
035 |a (NATIONALLICENCE)springer-10.1007/s00253-014-6293-3 
245 0 0 |a Response of denitrifying genes coding for nitrite ( nirK or nirS ) and nitrous oxide ( nosZ ) reductases to different physico-chemical parameters during agricultural waste composting  |h [Elektronische Daten]  |c [Lihua Zhang, Guangming Zeng, Jiachao Zhang, Yaoning Chen, Man Yu, Lunhui Lu, Hui Li, Yuan Zhu, Yujie Yuan, Aizhi Huang, Ling He] 
520 3 |a The present research was performed to clarify the changes of denitrifying genes (nirK, nirS, and nosZ) abundances under different physico-chemical parameters through evaluating the relationships between the genes abundances and parameters during agricultural waste composting. The genes abundances were determined by real-time quantitative PCR (qPCR). The correlations between physico-chemical parameters and denitrifying genes abundances were analysed by regression analysis. qPCR results showed that the nosZ gene abundance was higher than that of nirK and nirS genes. The nirK gene abundance was higher than nirS gene indicating that nitrite reducers with Cu-containing enzyme encoded by nirK gene were more of importance than those with cytochrome cd1 nitrite reductase encoded by nirS gene in the nitrite reduction step. Regression analysis suggested that (1) nirK gene abundance was correlated with pile temperature following quadratic model; (2) nirS gene abundance was linearly correlated with pile temperature and concentration of NH4 +, while correlated with concentration of NO3 − and pH following inverse and quadratic model respectively; (3) nosZ gene abundance was quadratically correlated with pH and linearly correlated with water soluble carbon (WSC). 
540 |a Springer-Verlag Berlin Heidelberg, 2015 
690 7 |a Composting  |2 nationallicence 
690 7 |a Denitrifying genes  |2 nationallicence 
690 7 |a Physico-chemical parameters  |2 nationallicence 
690 7 |a qPCR  |2 nationallicence 
690 7 |a Regression analysis  |2 nationallicence 
700 1 |a Zhang  |D Lihua  |u College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China  |4 aut 
700 1 |a Zeng  |D Guangming  |u College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China  |4 aut 
700 1 |a Zhang  |D Jiachao  |u College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China  |4 aut 
700 1 |a Chen  |D Yaoning  |u College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China  |4 aut 
700 1 |a Yu  |D Man  |u Environmental Resources and Soil Fertilizer Institute, Zhejiang Academy of Agricultural Sciences, 310021, Hangzhou, China  |4 aut 
700 1 |a Lu  |D Lunhui  |u College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China  |4 aut 
700 1 |a Li  |D Hui  |u Hunan Academy of Forestry and Biodiesel Engineering Research Centre of Hunan Province, 410004, Changsha, China  |4 aut 
700 1 |a Zhu  |D Yuan  |u College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China  |4 aut 
700 1 |a Yuan  |D Yujie  |u College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China  |4 aut 
700 1 |a Huang  |D Aizhi  |u College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China  |4 aut 
700 1 |a He  |D Ling  |u College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China  |4 aut 
773 0 |t Applied Microbiology and Biotechnology  |d Springer Berlin Heidelberg  |g 99/9(2015-05-01), 4059-4070  |x 0175-7598  |q 99:9<4059  |1 2015  |2 99  |o 253 
856 4 0 |u https://doi.org/10.1007/s00253-014-6293-3  |q text/html  |z Onlinezugriff via DOI 
898 |a BK010053  |b XK010053  |c XK010000 
900 7 |a Metadata rights reserved  |b Springer special CC-BY-NC licence  |2 nationallicence 
908 |D 1  |a research-article  |2 jats 
949 |B NATIONALLICENCE  |F NATIONALLICENCE  |b NL-springer 
950 |B NATIONALLICENCE  |P 856  |E 40  |u https://doi.org/10.1007/s00253-014-6293-3  |q text/html  |z Onlinezugriff via DOI 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Zhang  |D Lihua  |u College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Zeng  |D Guangming  |u College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Zhang  |D Jiachao  |u College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Chen  |D Yaoning  |u College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Yu  |D Man  |u Environmental Resources and Soil Fertilizer Institute, Zhejiang Academy of Agricultural Sciences, 310021, Hangzhou, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Lu  |D Lunhui  |u College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Li  |D Hui  |u Hunan Academy of Forestry and Biodiesel Engineering Research Centre of Hunan Province, 410004, Changsha, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Zhu  |D Yuan  |u College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Yuan  |D Yujie  |u College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Huang  |D Aizhi  |u College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a He  |D Ling  |u College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China  |4 aut 
950 |B NATIONALLICENCE  |P 773  |E 0-  |t Applied Microbiology and Biotechnology  |d Springer Berlin Heidelberg  |g 99/9(2015-05-01), 4059-4070  |x 0175-7598  |q 99:9<4059  |1 2015  |2 99  |o 253