caa a22 4500 605499934 CHVBK 20210128100553.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00253-014-6293-3 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6293-3 Response of denitrifying genes coding for nitrite ( nirK or nirS ) and nitrous oxide ( nosZ ) reductases to different physico-chemical parameters during agricultural waste composting [Elektronische Daten] [Lihua Zhang, Guangming Zeng, Jiachao Zhang, Yaoning Chen, Man Yu, Lunhui Lu, Hui Li, Yuan Zhu, Yujie Yuan, Aizhi Huang, Ling He] The present research was performed to clarify the changes of denitrifying genes (nirK, nirS, and nosZ) abundances under different physico-chemical parameters through evaluating the relationships between the genes abundances and parameters during agricultural waste composting. The genes abundances were determined by real-time quantitative PCR (qPCR). The correlations between physico-chemical parameters and denitrifying genes abundances were analysed by regression analysis. qPCR results showed that the nosZ gene abundance was higher than that of nirK and nirS genes. The nirK gene abundance was higher than nirS gene indicating that nitrite reducers with Cu-containing enzyme encoded by nirK gene were more of importance than those with cytochrome cd1 nitrite reductase encoded by nirS gene in the nitrite reduction step. Regression analysis suggested that (1) nirK gene abundance was correlated with pile temperature following quadratic model; (2) nirS gene abundance was linearly correlated with pile temperature and concentration of NH4 +, while correlated with concentration of NO3 − and pH following inverse and quadratic model respectively; (3) nosZ gene abundance was quadratically correlated with pH and linearly correlated with water soluble carbon (WSC). Springer-Verlag Berlin Heidelberg, 2015 Composting nationallicence Denitrifying genes nationallicence Physico-chemical parameters nationallicence qPCR nationallicence Regression analysis nationallicence Zhang Lihua College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut Zeng Guangming College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut Zhang Jiachao College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut Chen Yaoning College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut Yu Man Environmental Resources and Soil Fertilizer Institute, Zhejiang Academy of Agricultural Sciences, 310021, Hangzhou, China aut Lu Lunhui College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut Li Hui Hunan Academy of Forestry and Biodiesel Engineering Research Centre of Hunan Province, 410004, Changsha, China aut Zhu Yuan College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut Yuan Yujie College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut Huang Aizhi College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut He Ling College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/9(2015-05-01), 4059-4070 0175-7598 99:9<4059 2015 99 253 https://doi.org/10.1007/s00253-014-6293-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6293-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Lihua College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut NATIONALLICENCE 700 1- Zeng Guangming College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut NATIONALLICENCE 700 1- Zhang Jiachao College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut NATIONALLICENCE 700 1- Chen Yaoning College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut NATIONALLICENCE 700 1- Yu Man Environmental Resources and Soil Fertilizer Institute, Zhejiang Academy of Agricultural Sciences, 310021, Hangzhou, China aut NATIONALLICENCE 700 1- Lu Lunhui College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut NATIONALLICENCE 700 1- Li Hui Hunan Academy of Forestry and Biodiesel Engineering Research Centre of Hunan Province, 410004, Changsha, China aut NATIONALLICENCE 700 1- Zhu Yuan College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut NATIONALLICENCE 700 1- Yuan Yujie College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut NATIONALLICENCE 700 1- Huang Aizhi College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut NATIONALLICENCE 700 1- He Ling College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/9(2015-05-01), 4059-4070 0175-7598 99:9<4059 2015 99 253