Antimicrobial photodynamic therapy with fulleropyrrolidine: photoinactivation mechanism of Staphylococcus aureus , in vitro and in vivo studies
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6539-8 |2 doi |
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| 245 | 0 | 0 | |a Antimicrobial photodynamic therapy with fulleropyrrolidine: photoinactivation mechanism of Staphylococcus aureus , in vitro and in vivo studies |h [Elektronische Daten] |c [Mariusz Grinholc, Joanna Nakonieczna, Grzegorz Fila, Aleksandra Taraszkiewicz, Anna Kawiak, Grzegorz Szewczyk, Tadeusz Sarna, Lothar Lilge, Krzysztof Bielawski] |
| 520 | 3 | |a A family of N-methylpyrrolidinium fullerene iodide salts has been intensively studied to determine their applicability in antimicrobial photodynamic therapy (APDT). This study examined in vitro the efficacy of a C60 fullerene functionalized with one methylpyrrolidinium group to kill upon irradiation with white light gram-negative and gram-positive bacteria, as well as fungal cells, and the corresponding mechanism of the fullerene bactericidal action. The in vitro studies revealed that the high antistaphylococcal efficacy of functionalized fullerene could be linked to their ability to photogenerate singlet oxygen and superoxide anion. Following Staphylococcus aureus photoinactivation, no modifications of its genomic DNA were detected. In contrast, photodamage of the cell envelope seemed to be a dominant mechanism of bactericidal action. In in vivo studies, a 2 log10 reduction in the average bioluminescent radiance between treated and non-treated mice was reached. One day post APDT treatment, moist and abundant growth of bacteria could be observed on wounds of non-fulleropyrrolidine and dark control mice. APDT-treated wounds stayed visibly clear up to the third day. Moreover, cytotoxicity test on human dermal keratinocytes revealed great safety of using the sensitizer toward eukaryotic cells. These data indicate potential application of functionalized fullerene as antistaphylococcal sensitizer for superficial infections. | |
| 540 | |a The Author(s), 2015 | ||
| 690 | 7 | |a Cytotoxicity |2 nationallicence | |
| 690 | 7 | |a Fulleropyrrolidine |2 nationallicence | |
| 690 | 7 | |a Mouse model |2 nationallicence | |
| 690 | 7 | |a Photoinactivation |2 nationallicence | |
| 690 | 7 | |a Staphylococcus aureus |2 nationallicence | |
| 690 | 7 | |a Wound infection |2 nationallicence | |
| 700 | 1 | |a Grinholc |D Mariusz |u Laboratory of Molecular Diagnostics, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland |4 aut | |
| 700 | 1 | |a Nakonieczna |D Joanna |u Laboratory of Molecular Diagnostics, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland |4 aut | |
| 700 | 1 | |a Fila |D Grzegorz |u Laboratory of Molecular Diagnostics, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland |4 aut | |
| 700 | 1 | |a Taraszkiewicz |D Aleksandra |u Laboratory of Molecular Diagnostics, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland |4 aut | |
| 700 | 1 | |a Kawiak |D Anna |u Department of Biotechnology, Division of Plant Protection and Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland |4 aut | |
| 700 | 1 | |a Szewczyk |D Grzegorz |u Department of Biophysics, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387, Kraków, Poland |4 aut | |
| 700 | 1 | |a Sarna |D Tadeusz |u Department of Biophysics, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387, Kraków, Poland |4 aut | |
| 700 | 1 | |a Lilge |D Lothar |u Department of Medical Biophysics, Princess Margaret Cancer Centre/University of Toronto, 101 College Street 15-301, M5G 1 L7, Toronto, ON, Canada |4 aut | |
| 700 | 1 | |a Bielawski |D Krzysztof |u Laboratory of Molecular Diagnostics, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/9(2015-05-01), 4031-4043 |x 0175-7598 |q 99:9<4031 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6539-8 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6539-8 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Grinholc |D Mariusz |u Laboratory of Molecular Diagnostics, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Nakonieczna |D Joanna |u Laboratory of Molecular Diagnostics, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Fila |D Grzegorz |u Laboratory of Molecular Diagnostics, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Taraszkiewicz |D Aleksandra |u Laboratory of Molecular Diagnostics, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kawiak |D Anna |u Department of Biotechnology, Division of Plant Protection and Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Szewczyk |D Grzegorz |u Department of Biophysics, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387, Kraków, Poland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sarna |D Tadeusz |u Department of Biophysics, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387, Kraków, Poland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lilge |D Lothar |u Department of Medical Biophysics, Princess Margaret Cancer Centre/University of Toronto, 101 College Street 15-301, M5G 1 L7, Toronto, ON, Canada |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bielawski |D Krzysztof |u Laboratory of Molecular Diagnostics, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/9(2015-05-01), 4031-4043 |x 0175-7598 |q 99:9<4031 |1 2015 |2 99 |o 253 | ||