caa a22 4500 605499985 CHVBK 20210128100553.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00253-015-6484-6 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6484-6 Improving biohydrogen production using Clostridium beijerinckii immobilized with magnetite nanoparticles [Elektronische Daten] [Trevor Seelert, Dipankar Ghosh, Viviane Yargeau] In order to supplement the need for alternative energy resources within the near future, enhancing the production of biohydrogen with immobilized Clostridium beijerinckii NCIMB8052 was investigated. Magnetite nanoparticles were functionalized, with chitosan and alginic acid polyelectrolytes using a layer-by-layer method, to promote bacterial attachment. Cultivating C. beijerinckii with these nanoparticles resulted in a shorter lag growth phase and increased total biohydrogen production within 100-ml, 250-ml and 3.6-L reactors compared with freely suspended organisms. The greatest hydrogen yield was obtained in the 250-ml reactor with a value of 2.1 ± 0.7molH2/mol glucose, corresponding to substrate conversion and energy conversion efficiencies of 52 ± 18 and 10 ± 3%, respectively. The hydrogen yields obtained using the immobilized bacteria are comparable to values found in literature. However, to make this process viable, further improvements are required to increase the substrate and energy conversion efficiencies. Springer-Verlag Berlin Heidelberg, 2015 Biohydrogen nationallicence Clostridium beijerinckii nationallicence Magnetite nationallicence Chitosan nationallicence Alginic acid nationallicence Immobilization nationallicence Seelert Trevor Department of Chemical Engineering, McGill University, 3610 University St., H3A 2B2, Montréal, QC, Canada aut Ghosh Dipankar Département de Microbiologie et Immunologie, Université de Montréal, C.P. 6128, Succ. Centre-ville, H3C 3J7, Montréal, QC, Canada aut Yargeau Viviane Department of Chemical Engineering, McGill University, 3610 University St., H3A 2B2, Montréal, QC, Canada aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/9(2015-05-01), 4107-4116 0175-7598 99:9<4107 2015 99 253 https://doi.org/10.1007/s00253-015-6484-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6484-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Seelert Trevor Department of Chemical Engineering, McGill University, 3610 University St., H3A 2B2, Montréal, QC, Canada aut NATIONALLICENCE 700 1- Ghosh Dipankar Département de Microbiologie et Immunologie, Université de Montréal, C.P. 6128, Succ. Centre-ville, H3C 3J7, Montréal, QC, Canada aut NATIONALLICENCE 700 1- Yargeau Viviane Department of Chemical Engineering, McGill University, 3610 University St., H3A 2B2, Montréal, QC, Canada aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/9(2015-05-01), 4107-4116 0175-7598 99:9<4107 2015 99 253