Screening of a novel strong promoter by RNA sequencing and its application to H2 production in a hyperthermophilic archaeon
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6444-1 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6444-1 | ||
| 245 | 0 | 0 | |a Screening of a novel strong promoter by RNA sequencing and its application to H2 production in a hyperthermophilic archaeon |h [Elektronische Daten] |c [Seong Lee, Min-Sik Kim, Hae Jung, Jinwon Lee, Jung-Hyun Lee, Hyun Lee, Sung Kang] |
| 520 | 3 | |a A strong promoter increases transcription of the genes of interest and enhances the production of various valuable substances. For a hyperthermophilic archaeon Thermococcus onnurineus NA1, which can produce H2 from carbon monoxide oxidation, we searched for a novel endogenous strong promoter by transcriptome analysis using high-throughput RNA sequencing. Based on the relative transcript abundance, we selected one promoter to encode a hypothetical gene, of which homologs were found only in several Thermococcales strains. This promoter, P TN0510 , was introduced into the front of CO-responsible hydrogenase gene cluster encoding a carbon monoxide dehydrogenase (CODH), a hydrogenase, and a Na+/H+ antiporter. In the resulting mutant strain, KS0510, transcription and translation level of the gene cluster increased by 4- to 14-folds and 1.5- to 1.9-folds, respectively, in comparison with those of the wild-type strain. Additionally, H2 production rate of KS0510 mutant was 4.8-fold higher than that of the wild-type strain. The P TN0510 was identified to be much stronger than the well-known two strong promoters, gdh and slp promoters from Thermococcus strains, through RT-qPCR and Western blotting analyses and kinetics of H2 production. In this study, we demonstrated that the RNA-seq approach is a good strategy to mine novel strong promoters of use to a Thermococcus strain when developed as a biotechnologically promising strain to produce valuable products such as enzymes and metabolites through metabolic engineering. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Strong promoter |2 nationallicence | |
| 690 | 7 | |a RNA sequencing |2 nationallicence | |
| 690 | 7 | |a Thermococcus onnurineus NA1 |2 nationallicence | |
| 690 | 7 | |a Carbon monoxide |2 nationallicence | |
| 690 | 7 | |a H2 production |2 nationallicence | |
| 700 | 1 | |a Lee |D Seong |u Korea Institute of Ocean Science and Technology, Ansan, Korea |4 aut | |
| 700 | 1 | |a Kim |D Min-Sik |u Korea Institute of Ocean Science and Technology, Ansan, Korea |4 aut | |
| 700 | 1 | |a Jung |D Hae |u Korea Institute of Ocean Science and Technology, Ansan, Korea |4 aut | |
| 700 | 1 | |a Lee |D Jinwon |u Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, Korea |4 aut | |
| 700 | 1 | |a Lee |D Jung-Hyun |u Korea Institute of Ocean Science and Technology, Ansan, Korea |4 aut | |
| 700 | 1 | |a Lee |D Hyun |u Korea Institute of Ocean Science and Technology, Ansan, Korea |4 aut | |
| 700 | 1 | |a Kang |D Sung |u Korea Institute of Ocean Science and Technology, Ansan, Korea |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/9(2015-05-01), 4085-4092 |x 0175-7598 |q 99:9<4085 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6444-1 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6444-1 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lee |D Seong |u Korea Institute of Ocean Science and Technology, Ansan, Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kim |D Min-Sik |u Korea Institute of Ocean Science and Technology, Ansan, Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Jung |D Hae |u Korea Institute of Ocean Science and Technology, Ansan, Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lee |D Jinwon |u Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lee |D Jung-Hyun |u Korea Institute of Ocean Science and Technology, Ansan, Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lee |D Hyun |u Korea Institute of Ocean Science and Technology, Ansan, Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kang |D Sung |u Korea Institute of Ocean Science and Technology, Ansan, Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/9(2015-05-01), 4085-4092 |x 0175-7598 |q 99:9<4085 |1 2015 |2 99 |o 253 | ||