caa a22 4500 605500037 CHVBK 20210128100554.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00253-014-6332-0 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6332-0 Construction and evaluation of a fluorescence-based live attenuated Escherichia coli delivery system for generating oral vaccine candidate [Elektronische Daten] [Wenxin Liu, Xingyue Li, Jun Bao, Weikun Guan, Zhiteng Zhao, Chaowen Yuan, Jie Tang, Dongfang Shi] Enter toxigenic Escherichia coli (ETEC) is a major pathogen of swine industry that can have a substantial impact on morbidity and mortality. Therefore, it is necessary to develop effective vaccines for the prevention of ETEC infection. Live attenuated bacteria delivery system are effective tools for mucosal immunization. The purpose of this study was to construct a novel delivery system that can present the LTR192G-STb fusion protein as oral vaccine candidate. Firstly, the PRPL-mKate2 fluorescent cassette was inserted into the genome (yaiT pseudogene) of an attenuated E. coli by homologous recombination methods to construct the delivery system O142(yaiT::PRPL-mKate2). Secondly, the oral vaccine O142(yaiT:: LT192-STb) (ER-B) was derived for replacing the PRPL-mKate2 by LT192-STb fusion gene, and then it was tested for its feasibility as oral vaccine candidate. Subsequently, BALB/c mice were orogastrically immunized with ER-B. Results showed that mice orally immunized with ER-B produced high levels of specific IgA and IgG antibodies. The induced antibodies demonstrated neutralizing effects to enter toxins LT and STb. In addition, results of cellular immune responses showed that stimulation index values of immunized mice were significantly higher than the control group (P < 0.05) and with a marked shift towards Th 2 immunity. These data indicated that the recombinant E. coli ER-B could be a valuable candidate of future vaccines against ETEC infection. Springer-Verlag Berlin Heidelberg, 2015 Escherichia coli nationallicence Mucosal vaccine nationallicence Mice nationallicence Enterotoxin nationallicence Liu Wenxin Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut Li Xingyue Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut Bao Jun College of Animal Science and Technology, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut Guan Weikun Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut Zhao Zhiteng Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut Yuan Chaowen Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut Tang Jie Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut Shi Dongfang Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/9(2015-05-01), 4005-4018 0175-7598 99:9<4005 2015 99 253 https://doi.org/10.1007/s00253-014-6332-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6332-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Wenxin Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut NATIONALLICENCE 700 1- Li Xingyue Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut NATIONALLICENCE 700 1- Bao Jun College of Animal Science and Technology, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut NATIONALLICENCE 700 1- Guan Weikun Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut NATIONALLICENCE 700 1- Zhao Zhiteng Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut NATIONALLICENCE 700 1- Yuan Chaowen Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut NATIONALLICENCE 700 1- Tang Jie Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut NATIONALLICENCE 700 1- Shi Dongfang Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/9(2015-05-01), 4005-4018 0175-7598 99:9<4005 2015 99 253