Simultaneous oral immunization of mice with live attenuated Escherichia coli expressing LT192-STa13 and LT192-STb fusion immunogen, respectively, for polyvalent vaccine candidate
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6302-6 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6302-6 | ||
| 245 | 0 | 0 | |a Simultaneous oral immunization of mice with live attenuated Escherichia coli expressing LT192-STa13 and LT192-STb fusion immunogen, respectively, for polyvalent vaccine candidate |h [Elektronische Daten] |c [Wenxin Liu, Jinping Li, Jun Bao, Xingyue Li, Weikun Guan, Chaowen Yuan, Jie Tang, Zhiteng Zhao, Dongfang Shi] |
| 520 | 3 | |a Previous epidemiological study showed that most of the porcine enterotoxin Escherichia coli (ETEC) strains harbor multiple enterotoxins but lack any of the fimbriae or non-fimbrial adhesion genes. Therefore, effective ETEC vaccines need to aim directly at all the enterotoxin antigens. The objective of this study was to evaluate the simultaneous immune effect of two live attenuated E. coli strains expressing LTR192G-STaA13Q and LTR192G-STb fusion immunogen, respectively. The results showed that both local mucosal and systemic immune responses against LT, STa, STb, and F41 were induced in BALB/c mice immunized orally with the recombinant E. coli strains ER-A and ER-B simultaneously. In addition, results of cellular immune responses showed that stimulation index (SI) values of immunized mice were significantly higher than control mice (P < 0.05) and a marked shift toward type-2 helper T lymphocyte (Th 2) immunity. Moreover, the induced antibodies demonstrated neutralizing effects on LT, STa, and STb producing E. coli infection. These data indicated that the use of recombinant E. coli ER-A and ER-B could be a valuable strategy for future polyvalent vaccine development of ETEC. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Escherichia coli |2 nationallicence | |
| 690 | 7 | |a Mucosal vaccine |2 nationallicence | |
| 690 | 7 | |a Mice |2 nationallicence | |
| 690 | 7 | |a ETEC |2 nationallicence | |
| 690 | 7 | |a Polyvalent vaccine |2 nationallicence | |
| 700 | 1 | |a Liu |D Wenxin |u Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China |4 aut | |
| 700 | 1 | |a Li |D Jinping |u Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China |4 aut | |
| 700 | 1 | |a Bao |D Jun |u College of Animal Science and Technology, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China |4 aut | |
| 700 | 1 | |a Li |D Xingyue |u Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China |4 aut | |
| 700 | 1 | |a Guan |D Weikun |u Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China |4 aut | |
| 700 | 1 | |a Yuan |D Chaowen |u Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China |4 aut | |
| 700 | 1 | |a Tang |D Jie |u Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China |4 aut | |
| 700 | 1 | |a Zhao |D Zhiteng |u Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China |4 aut | |
| 700 | 1 | |a Shi |D Dongfang |u Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/9(2015-05-01), 3981-3992 |x 0175-7598 |q 99:9<3981 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6302-6 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6302-6 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Liu |D Wenxin |u Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Li |D Jinping |u Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bao |D Jun |u College of Animal Science and Technology, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Li |D Xingyue |u Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Guan |D Weikun |u Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yuan |D Chaowen |u Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Tang |D Jie |u Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhao |D Zhiteng |u Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Shi |D Dongfang |u Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/9(2015-05-01), 3981-3992 |x 0175-7598 |q 99:9<3981 |1 2015 |2 99 |o 253 | ||