caa a22 4500 605500053 CHVBK 20210128100554.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00253-014-6302-6 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6302-6 Simultaneous oral immunization of mice with live attenuated Escherichia coli expressing LT192-STa13 and LT192-STb fusion immunogen, respectively, for polyvalent vaccine candidate [Elektronische Daten] [Wenxin Liu, Jinping Li, Jun Bao, Xingyue Li, Weikun Guan, Chaowen Yuan, Jie Tang, Zhiteng Zhao, Dongfang Shi] Previous epidemiological study showed that most of the porcine enterotoxin Escherichia coli (ETEC) strains harbor multiple enterotoxins but lack any of the fimbriae or non-fimbrial adhesion genes. Therefore, effective ETEC vaccines need to aim directly at all the enterotoxin antigens. The objective of this study was to evaluate the simultaneous immune effect of two live attenuated E. coli strains expressing LTR192G-STaA13Q and LTR192G-STb fusion immunogen, respectively. The results showed that both local mucosal and systemic immune responses against LT, STa, STb, and F41 were induced in BALB/c mice immunized orally with the recombinant E. coli strains ER-A and ER-B simultaneously. In addition, results of cellular immune responses showed that stimulation index (SI) values of immunized mice were significantly higher than control mice (P < 0.05) and a marked shift toward type-2 helper T lymphocyte (Th 2) immunity. Moreover, the induced antibodies demonstrated neutralizing effects on LT, STa, and STb producing E. coli infection. These data indicated that the use of recombinant E. coli ER-A and ER-B could be a valuable strategy for future polyvalent vaccine development of ETEC. Springer-Verlag Berlin Heidelberg, 2014 Escherichia coli nationallicence Mucosal vaccine nationallicence Mice nationallicence ETEC nationallicence Polyvalent vaccine nationallicence Liu Wenxin Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut Li Jinping Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut Bao Jun College of Animal Science and Technology, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut Li Xingyue Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut Guan Weikun Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut Yuan Chaowen Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut Tang Jie Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut Zhao Zhiteng Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut Shi Dongfang Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/9(2015-05-01), 3981-3992 0175-7598 99:9<3981 2015 99 253 https://doi.org/10.1007/s00253-014-6302-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6302-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Wenxin Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut NATIONALLICENCE 700 1- Li Jinping Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut NATIONALLICENCE 700 1- Bao Jun College of Animal Science and Technology, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut NATIONALLICENCE 700 1- Li Xingyue Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut NATIONALLICENCE 700 1- Guan Weikun Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut NATIONALLICENCE 700 1- Yuan Chaowen Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut NATIONALLICENCE 700 1- Tang Jie Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut NATIONALLICENCE 700 1- Zhao Zhiteng Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut NATIONALLICENCE 700 1- Shi Dongfang Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/9(2015-05-01), 3981-3992 0175-7598 99:9<3981 2015 99 253