caa a22 4500 605500061 CHVBK 20210128100554.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00253-014-6292-4 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6292-4 Effect of 1,3-propanediol, organic acids, and ethanol on growth and metabolism of Clostridium butyricum DSP1 [Elektronische Daten] [Daria Szymanowska-Powałowska, Piotr Kubiak] Knowledge of tolerance of bacteria to toxic stress is important, especially for processes targeted at high final titers of product. Information on environmental limits and stress responses may help during selection of strains or design and control of processes. The influence of the main product and its co-products on the process of 1,3-propanediol (PD) synthesis was determined. Adaptation to toxic compounds was noticed as Clostridium butyricum DSP1 was less sensitive to the addition of these factors during its exponential growth on glycerol than when the factor was present in the medium before inoculation. It was also shown that the response of the tested strain to the toxicity of 1,3-propanediol (1,3-PD) has different proteomic profiles depending on the stage of culture when this substance is introduced. Relatively satisfactory activity of the analyzed strain was sustained up to a concentration of 1,3-PD of 40g/L while 80g/L of this metabolite was lethal to the bacterium. As for the by-products, acetic acid was determined to be the most toxic among the acids excreted during the process. Springer-Verlag Berlin Heidelberg, 2014 Clostridium butyricum nationallicence Environmental stress nationallicence Ethanol nationallicence Organic acids nationallicence 1,3-Propanediol nationallicence Szymanowska-Powałowska Daria Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48, 60-527, Poznan, Poland aut Kubiak Piotr Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48, 60-527, Poznan, Poland aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 3179-3189 0175-7598 99:7<3179 2015 99 253 https://doi.org/10.1007/s00253-014-6292-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6292-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Szymanowska-Powałowska Daria Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48, 60-527, Poznan, Poland aut NATIONALLICENCE 700 1- Kubiak Piotr Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48, 60-527, Poznan, Poland aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 3179-3189 0175-7598 99:7<3179 2015 99 253