caa a22 4500 60550007X CHVBK 20210128100554.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00253-015-6498-0 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6498-0 Lactic acid bacteria—20years exploring their potential as live vectors for mucosal vaccination [Elektronische Daten] [Agnieszka Wyszyńska, Patrycja Kobierecka, Jacek Bardowski, Elżbieta Jagusztyn-Krynicka] Lactic acid bacteria (LAB) are a diverse group of Gram-positive, nonsporulating, low G + C content bacteria. Many of them have been given generally regarded as safe status. Over the past two decades, intensive genetic and molecular research carried out on LAB, mainly Lactococcus lactis and some species of the Lactobacillus genus, has revealed new, potential biomedical LAB applications, including the use of LAB as adjuvants, immunostimulators, or therapeutic drug delivery systems, or as factories to produce therapeutic molecules. LAB enable immunization via the mucosal route, which increases effectiveness against pathogens that use the mucosa as the major route of entry into the human body. In this review, we concentrate on the encouraging application of Lactococcus and Lactobacillus genera for the development of live mucosal vaccines. First, we present the progress that has recently been made in the field of developing tools for LAB genetic manipulations, which has resulted in the successful expression of many bacterial, parasitic, and viral antigens in LAB strains. Next, we discuss the factors influencing the efficacy of the constructed vaccine prototypes that have been tested in various animal models. Apart from the research focused on an application of live LABs as carriers of foreign antigens, a lot of work has been recently done on the potential usage of nonliving, nonrecombinant L. lactis designated as Gram-positive enhancer matrix (GEM), as a delivery system for mucosal vaccination. The advantages and disadvantages of both strategies are also presented. The Author(s), 2015 Lactic acid bacteria nationallicence Antigens nationallicence DNA vaccine nationallicence GEM particles nationallicence Immunoprophylaxis nationallicence Wyszyńska Agnieszka Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland aut Kobierecka Patrycja Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland aut Bardowski Jacek Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106, Warsaw, Poland aut Jagusztyn-Krynicka Elżbieta Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 2967-2977 0175-7598 99:7<2967 2015 99 253 https://doi.org/10.1007/s00253-015-6498-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6498-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wyszyńska Agnieszka Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland aut NATIONALLICENCE 700 1- Kobierecka Patrycja Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland aut NATIONALLICENCE 700 1- Bardowski Jacek Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106, Warsaw, Poland aut NATIONALLICENCE 700 1- Jagusztyn-Krynicka Elżbieta Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 2967-2977 0175-7598 99:7<2967 2015 99 253