caa a22 4500 605500096 CHVBK 20210128100554.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00253-014-6222-5 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6222-5 Change of microbial community structure and functional gene abundance in nonylphenol-degrading sediment [Elektronische Daten] [Zhao Wang, Yuyin Yang, Tao He, Shuguang Xie] Biodegradation by autochthonous microbial community is an important way to clean up nonylphenol (NP) from contaminated river sediment. Knowledge of sediment microbial community can aid in our understanding of biological processes related to NP degradation. However, the change in sediment microbial community associated with NP biodegradation remains unclear. The present study investigated the shift in bacterial community structure and NP-degrading gene abundance in response to NP attenuation in river sediment. Sediment microcosms with different levels of 4-NP (0, 100, or 300μg/g) were constructed. A nearly complete attenuation of NP occurred in the microcosm with 100μg/g NP after 9days' incubation, while a residual NP rate of 8.1% was observed in the microcosm with 300μg/g NP after 22days' incubation. Illumina MiSeq sequencing analysis indicated that Gammaproteobacteria, Alphaproteobacteria, and Bacteroidetes predominated in NP-degrading river sediment. Sediment bacterial community structure varied significantly during NP biodegradation and subsequent incubation, which was affected by the level of added NP. The n-alkane biodegradation (alkB) gene abundance showed a significant variation in each NP-amended microcosm (100 or 300μg/g), while a significant increase in the single component monooxygenase (sMO) gene abundance only occurred in the microcosm spiked with 300μg/g NP. This study can provide some new insights toward NP-degrading microbial ecology in the environment. Springer-Verlag Berlin Heidelberg, 2014 Biodegradation nationallicence Bacterial community nationallicence alkB nationallicence sMO nationallicence Illumina MiSeq sequencing nationallicence Quantitative PCR nationallicence Wang Zhao State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China aut Yang Yuyin State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China aut He Tao South China Institute of Environmental Sciences, Ministry of Environment Protection (MEP), 510655, Guangzhou, China aut Xie Shuguang State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 3259-3268 0175-7598 99:7<3259 2015 99 253 https://doi.org/10.1007/s00253-014-6222-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6222-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Zhao State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China aut NATIONALLICENCE 700 1- Yang Yuyin State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China aut NATIONALLICENCE 700 1- He Tao South China Institute of Environmental Sciences, Ministry of Environment Protection (MEP), 510655, Guangzhou, China aut NATIONALLICENCE 700 1- Xie Shuguang State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 3259-3268 0175-7598 99:7<3259 2015 99 253