Quo vadis? The challenges of recombinant protein folding and secretion in Pichia pastoris
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6470-z |2 doi |
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| 245 | 0 | 0 | |a Quo vadis? The challenges of recombinant protein folding and secretion in Pichia pastoris |h [Elektronische Daten] |c [Verena Puxbaum, Diethard Mattanovich, Brigitte Gasser] |
| 520 | 3 | |a The development of Pichia pastoris as a production platform for recombinant proteins has been a remarkable success story over the last three decades. Stable cheap production processes and the good protein secretion abilities were pacemakers of this development. However, limitations of protein folding, glycosylation or secretion have been identified quite early on. With the availability of genome sequences and the development of systems biology characterization in the last 5years, remarkable success in strain improvement was achieved. Here, we focus on recent developments of characterization and improvement of P. pastoris production strains regarding protein folding, intracellular trafficking, glycosylation and proteolytic degradation. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Yeast |2 nationallicence | |
| 690 | 7 | |a Pichia pastoris |2 nationallicence | |
| 690 | 7 | |a Recombinant protein |2 nationallicence | |
| 690 | 7 | |a Folding |2 nationallicence | |
| 690 | 7 | |a Secretion |2 nationallicence | |
| 690 | 7 | |a Glycosylation |2 nationallicence | |
| 700 | 1 | |a Puxbaum |D Verena |u Austrian Centre of Industrial Biotechnology, Muthgasse 11, 1190, Vienna, Austria |4 aut | |
| 700 | 1 | |a Mattanovich |D Diethard |u Austrian Centre of Industrial Biotechnology, Muthgasse 11, 1190, Vienna, Austria |4 aut | |
| 700 | 1 | |a Gasser |D Brigitte |u Austrian Centre of Industrial Biotechnology, Muthgasse 11, 1190, Vienna, Austria |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/7(2015-04-01), 2925-2938 |x 0175-7598 |q 99:7<2925 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6470-z |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a review-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6470-z |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Puxbaum |D Verena |u Austrian Centre of Industrial Biotechnology, Muthgasse 11, 1190, Vienna, Austria |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Mattanovich |D Diethard |u Austrian Centre of Industrial Biotechnology, Muthgasse 11, 1190, Vienna, Austria |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Gasser |D Brigitte |u Austrian Centre of Industrial Biotechnology, Muthgasse 11, 1190, Vienna, Austria |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/7(2015-04-01), 2925-2938 |x 0175-7598 |q 99:7<2925 |1 2015 |2 99 |o 253 | ||