caa a22 4500 605500126 CHVBK 20210128100554.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00253-015-6470-z doi (NATIONALLICENCE)springer-10.1007/s00253-015-6470-z Quo vadis? The challenges of recombinant protein folding and secretion in Pichia pastoris [Elektronische Daten] [Verena Puxbaum, Diethard Mattanovich, Brigitte Gasser] The development of Pichia pastoris as a production platform for recombinant proteins has been a remarkable success story over the last three decades. Stable cheap production processes and the good protein secretion abilities were pacemakers of this development. However, limitations of protein folding, glycosylation or secretion have been identified quite early on. With the availability of genome sequences and the development of systems biology characterization in the last 5years, remarkable success in strain improvement was achieved. Here, we focus on recent developments of characterization and improvement of P. pastoris production strains regarding protein folding, intracellular trafficking, glycosylation and proteolytic degradation. Springer-Verlag Berlin Heidelberg, 2015 Yeast nationallicence Pichia pastoris nationallicence Recombinant protein nationallicence Folding nationallicence Secretion nationallicence Glycosylation nationallicence Puxbaum Verena Austrian Centre of Industrial Biotechnology, Muthgasse 11, 1190, Vienna, Austria aut Mattanovich Diethard Austrian Centre of Industrial Biotechnology, Muthgasse 11, 1190, Vienna, Austria aut Gasser Brigitte Austrian Centre of Industrial Biotechnology, Muthgasse 11, 1190, Vienna, Austria aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 2925-2938 0175-7598 99:7<2925 2015 99 253 https://doi.org/10.1007/s00253-015-6470-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6470-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Puxbaum Verena Austrian Centre of Industrial Biotechnology, Muthgasse 11, 1190, Vienna, Austria aut NATIONALLICENCE 700 1- Mattanovich Diethard Austrian Centre of Industrial Biotechnology, Muthgasse 11, 1190, Vienna, Austria aut NATIONALLICENCE 700 1- Gasser Brigitte Austrian Centre of Industrial Biotechnology, Muthgasse 11, 1190, Vienna, Austria aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 2925-2938 0175-7598 99:7<2925 2015 99 253