Hyperthermophilic aldolases as biocatalyst for C-C bond formation: rhamnulose 1-phosphate aldolase from Thermotoga maritima
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6123-7 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6123-7 | ||
| 245 | 0 | 0 | |a Hyperthermophilic aldolases as biocatalyst for C-C bond formation: rhamnulose 1-phosphate aldolase from Thermotoga maritima |h [Elektronische Daten] |c [Isabel Oroz-Guinea, Israel Sánchez-Moreno, Montaña Mena, Eduardo García-Junceda] |
| 520 | 3 | |a The TM1072 gene from Thermotoga maritima codifies for a putative form of a rhamnulose-1-phosphate aldolase (Rha-1PA Tm). To investigate this enzyme further, its gene was cloned and expressed in Escherichia coli. The purified enzyme was activated by Co2+ as a divalent metal ion cofactor, instead of Zn2+ as its E. coli homologue, and exhibited a maximum of activity at 95°C. Furthermore, the enzyme displayed a high stability against extreme reaction conditions, retaining 90% of its activity in the presence of 40% of acetonitrile and showing a half-life greater than 3h at 115°C. The kinetic parameters at room temperature (R/T) were also studied; the K M was calculated to be 3.6 ± 0.33mM, while k cat/K M was found to be 0.7 × 103s−1M−1. Given these characteristics, Rha-1PA Tm is an attractive enzyme for use as a biocatalyst for industrial applications, offering intriguing possibilities for practical biocatalysis. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Aldolases |2 nationallicence | |
| 690 | 7 | |a Biocatalysis |2 nationallicence | |
| 690 | 7 | |a Enzyme catalysis |2 nationallicence | |
| 690 | 7 | |a Hyperthermophilic enzymes |2 nationallicence | |
| 690 | 7 | |a Thermozyme |2 nationallicence | |
| 690 | 7 | |a Thermostability |2 nationallicence | |
| 700 | 1 | |a Oroz-Guinea |D Isabel |u Departamento de Química Bioorgánica, Instituto de Química Orgánica General, CSIC, Juan de la Cierva 3, 28006, Madrid, Spain |4 aut | |
| 700 | 1 | |a Sánchez-Moreno |D Israel |u Departamento de Química Bioorgánica, Instituto de Química Orgánica General, CSIC, Juan de la Cierva 3, 28006, Madrid, Spain |4 aut | |
| 700 | 1 | |a Mena |D Montaña |u Departamento de Ciencias Ambientales, Facultad de Ciencias Ambientales y Bioquímica, UCLM, Avda. Carlos III s/n, 45071, Toledo, Spain |4 aut | |
| 700 | 1 | |a García-Junceda |D Eduardo |u Departamento de Química Bioorgánica, Instituto de Química Orgánica General, CSIC, Juan de la Cierva 3, 28006, Madrid, Spain |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/7(2015-04-01), 3057-3068 |x 0175-7598 |q 99:7<3057 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6123-7 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6123-7 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Oroz-Guinea |D Isabel |u Departamento de Química Bioorgánica, Instituto de Química Orgánica General, CSIC, Juan de la Cierva 3, 28006, Madrid, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sánchez-Moreno |D Israel |u Departamento de Química Bioorgánica, Instituto de Química Orgánica General, CSIC, Juan de la Cierva 3, 28006, Madrid, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Mena |D Montaña |u Departamento de Ciencias Ambientales, Facultad de Ciencias Ambientales y Bioquímica, UCLM, Avda. Carlos III s/n, 45071, Toledo, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a García-Junceda |D Eduardo |u Departamento de Química Bioorgánica, Instituto de Química Orgánica General, CSIC, Juan de la Cierva 3, 28006, Madrid, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/7(2015-04-01), 3057-3068 |x 0175-7598 |q 99:7<3057 |1 2015 |2 99 |o 253 | ||