caa a22 4500 605500142 CHVBK 20210128100554.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00253-014-6204-7 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6204-7 Bacterial community shift along with the changes in operational conditions in a membrane-aerated biofilm reactor [Elektronische Daten] [Hai-Long Tian, Jie-Yu Zhao, Hong-Yu Zhang, Chang-Qiao Chi, Bao-An Li, Xiao-Lei Wu] Membrane-aerated biofilm reactor (MABR) is a promising wastewater treatment process. Although bacteria inhabiting the MABR biofilm are important in wastewater treatment, the community composition and its correlation with operating conditions were less clear. A laboratory-scale MABR was designed to investigate the shift of bacterial community through a complete operational process by pyrosequencing the bacterial 16S rRNA genes. From around 19,000 sequences, 175 bacterial genera were retrieved, mainly belonging to Betaproteobacteria, Gammaproteobacteria, Alphaproteobacteria, Bacteroidetes, and Actinobacteria. A large number of unclassified bacterial sequences were also detected in the biofilm, suggesting a wide variety of uncharacterized species in MABR. Redundancy analysis (RDA) revealed that influent chemical oxygen demand (COD), NH4-N, and NaHCO3 concentrations could exert distinct influences on the composition of the bacterial community. The influent COD and NaHCO3 concentrations stimulated proliferation of denitrification-related species such as Dokdonella, Azospira, Hydrogenophaga, Rhodocyclaceae, and Thauera, while inhibiting the growth of Acidovorax and Sinobacteraceae. Some denitrifying Thermomonas spp. tended to survive in NH4-N-rich environments, while Flavobacterium preferred to inhabit NH4-N-poor or COD-rich environments. Conversely, the influent NH4-N and NaHCO3, to some extent, appeared to be the growth-promoting factors for nitrifying bacteria. Furthermore, the presence of potential aerobic denitrifiers such as Comamonas, Enterobacter, and Aeromonas indicated that MABR could have the capability of simultaneous aerobic and anoxic denitrification particularly during treatment of low-ammonia nitrogen sewage. Springer-Verlag Berlin Heidelberg, 2014 MABR nationallicence Biofilm nationallicence Bacterial community structure nationallicence 454-pyrosequencing nationallicence Tian Hai-Long Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, 300072, Tianjin, People's Republic of China aut Zhao Jie-Yu Department of Energy and Resources Engineering, College of Engineering, Peking University, 100871, Beijing, People's Republic of China aut Zhang Hong-Yu Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, 300072, Tianjin, People's Republic of China aut Chi Chang-Qiao Department of Energy and Resources Engineering, College of Engineering, Peking University, 100871, Beijing, People's Republic of China aut Li Bao-An Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, 300072, Tianjin, People's Republic of China aut Wu Xiao-Lei Department of Energy and Resources Engineering, College of Engineering, Peking University, 100871, Beijing, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 3279-3290 0175-7598 99:7<3279 2015 99 253 https://doi.org/10.1007/s00253-014-6204-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6204-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tian Hai-Long Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, 300072, Tianjin, People's Republic of China aut NATIONALLICENCE 700 1- Zhao Jie-Yu Department of Energy and Resources Engineering, College of Engineering, Peking University, 100871, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Hong-Yu Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, 300072, Tianjin, People's Republic of China aut NATIONALLICENCE 700 1- Chi Chang-Qiao Department of Energy and Resources Engineering, College of Engineering, Peking University, 100871, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Li Bao-An Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, 300072, Tianjin, People's Republic of China aut NATIONALLICENCE 700 1- Wu Xiao-Lei Department of Energy and Resources Engineering, College of Engineering, Peking University, 100871, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 3279-3290 0175-7598 99:7<3279 2015 99 253