Increased production of free fatty acids in Aspergillus oryzae by disruption of a predicted acyl-CoA synthetase gene
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6336-9 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6336-9 | ||
| 245 | 0 | 0 | |a Increased production of free fatty acids in Aspergillus oryzae by disruption of a predicted acyl-CoA synthetase gene |h [Elektronische Daten] |c [Koichi Tamano, Kenneth Bruno, Hideaki Koike, Tomoko Ishii, Ai Miura, Myco Umemura, David Culley, Scott Baker, Masayuki Machida] |
| 520 | 3 | |a Fatty acids are attractive molecules as source materials for the production of biodiesel fuel. Previously, we attained a 2.4-fold increase in fatty acid production by increasing the expression of fatty acid synthesis-related genes in Aspergillus oryzae. In this study, we achieved an additional increase in the production of fatty acids by disrupting a predicted acyl-CoA synthetase gene in A. oryzae. The A. oryzae genome is predicted to encode six acyl-CoA synthetase genes and disruption of AO090011000642, one of the six genes, resulted in a 9.2-fold higher accumulation (corresponding to an increased production of 0.23mmol/g dry cell weight) of intracellular fatty acid in comparison to the wild-type strain. Furthermore, by introducing a niaD marker from Aspergillus nidulans to the disruptant, as well as changing the concentration of nitrogen in the culture medium from 10 to 350mM, fatty acid productivity reached 0.54mmol/g dry cell weight. Analysis of the relative composition of the major intracellular free fatty acids caused by disruption of AO090011000642 in comparison to the wild-type strain showed an increase in stearic acid (7 to 26%), decrease in linoleic acid (50 to 27%), and no significant changes in palmitic or oleic acid (each around 20-25%). | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Acyl-CoA synthetase |2 nationallicence | |
| 690 | 7 | |a Fatty acid |2 nationallicence | |
| 690 | 7 | |a Biofuel |2 nationallicence | |
| 690 | 7 | |a Productivity |2 nationallicence | |
| 690 | 7 | |a Genetic engineering |2 nationallicence | |
| 690 | 7 | |a Aspergillus oryzae |2 nationallicence | |
| 700 | 1 | |a Tamano |D Koichi |u Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, 062-8517, Sapporo, Hokkaido, Japan |4 aut | |
| 700 | 1 | |a Bruno |D Kenneth |u Chemical and Biological Process Development Group, Pacific Northwest National Laboratory, 902 Battelle Boulevard, 99352, Richland, WA, USA |4 aut | |
| 700 | 1 | |a Koike |D Hideaki |u Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, 305-8566, Tsukuba, Ibaraki, Japan |4 aut | |
| 700 | 1 | |a Ishii |D Tomoko |u Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, 062-8517, Sapporo, Hokkaido, Japan |4 aut | |
| 700 | 1 | |a Miura |D Ai |u Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, 062-8517, Sapporo, Hokkaido, Japan |4 aut | |
| 700 | 1 | |a Umemura |D Myco |u Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, 062-8517, Sapporo, Hokkaido, Japan |4 aut | |
| 700 | 1 | |a Culley |D David |u Chemical and Biological Process Development Group, Pacific Northwest National Laboratory, 902 Battelle Boulevard, 99352, Richland, WA, USA |4 aut | |
| 700 | 1 | |a Baker |D Scott |u Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, 3335 Innovation Boulevard, 99354, Richland, WA, USA |4 aut | |
| 700 | 1 | |a Machida |D Masayuki |u Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, 062-8517, Sapporo, Hokkaido, Japan |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/7(2015-04-01), 3103-3113 |x 0175-7598 |q 99:7<3103 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6336-9 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6336-9 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Tamano |D Koichi |u Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, 062-8517, Sapporo, Hokkaido, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bruno |D Kenneth |u Chemical and Biological Process Development Group, Pacific Northwest National Laboratory, 902 Battelle Boulevard, 99352, Richland, WA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Koike |D Hideaki |u Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, 305-8566, Tsukuba, Ibaraki, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ishii |D Tomoko |u Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, 062-8517, Sapporo, Hokkaido, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Miura |D Ai |u Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, 062-8517, Sapporo, Hokkaido, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Umemura |D Myco |u Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, 062-8517, Sapporo, Hokkaido, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Culley |D David |u Chemical and Biological Process Development Group, Pacific Northwest National Laboratory, 902 Battelle Boulevard, 99352, Richland, WA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Baker |D Scott |u Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, 3335 Innovation Boulevard, 99354, Richland, WA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Machida |D Masayuki |u Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, 062-8517, Sapporo, Hokkaido, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/7(2015-04-01), 3103-3113 |x 0175-7598 |q 99:7<3103 |1 2015 |2 99 |o 253 | ||