caa a22 4500 605500185 CHVBK 20210128100554.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00253-014-6336-9 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6336-9 Increased production of free fatty acids in Aspergillus oryzae by disruption of a predicted acyl-CoA synthetase gene [Elektronische Daten] [Koichi Tamano, Kenneth Bruno, Hideaki Koike, Tomoko Ishii, Ai Miura, Myco Umemura, David Culley, Scott Baker, Masayuki Machida] Fatty acids are attractive molecules as source materials for the production of biodiesel fuel. Previously, we attained a 2.4-fold increase in fatty acid production by increasing the expression of fatty acid synthesis-related genes in Aspergillus oryzae. In this study, we achieved an additional increase in the production of fatty acids by disrupting a predicted acyl-CoA synthetase gene in A. oryzae. The A. oryzae genome is predicted to encode six acyl-CoA synthetase genes and disruption of AO090011000642, one of the six genes, resulted in a 9.2-fold higher accumulation (corresponding to an increased production of 0.23mmol/g dry cell weight) of intracellular fatty acid in comparison to the wild-type strain. Furthermore, by introducing a niaD marker from Aspergillus nidulans to the disruptant, as well as changing the concentration of nitrogen in the culture medium from 10 to 350mM, fatty acid productivity reached 0.54mmol/g dry cell weight. Analysis of the relative composition of the major intracellular free fatty acids caused by disruption of AO090011000642 in comparison to the wild-type strain showed an increase in stearic acid (7 to 26%), decrease in linoleic acid (50 to 27%), and no significant changes in palmitic or oleic acid (each around 20-25%). Springer-Verlag Berlin Heidelberg, 2015 Acyl-CoA synthetase nationallicence Fatty acid nationallicence Biofuel nationallicence Productivity nationallicence Genetic engineering nationallicence Aspergillus oryzae nationallicence Tamano Koichi Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, 062-8517, Sapporo, Hokkaido, Japan aut Bruno Kenneth Chemical and Biological Process Development Group, Pacific Northwest National Laboratory, 902 Battelle Boulevard, 99352, Richland, WA, USA aut Koike Hideaki Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, 305-8566, Tsukuba, Ibaraki, Japan aut Ishii Tomoko Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, 062-8517, Sapporo, Hokkaido, Japan aut Miura Ai Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, 062-8517, Sapporo, Hokkaido, Japan aut Umemura Myco Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, 062-8517, Sapporo, Hokkaido, Japan aut Culley David Chemical and Biological Process Development Group, Pacific Northwest National Laboratory, 902 Battelle Boulevard, 99352, Richland, WA, USA aut Baker Scott Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, 3335 Innovation Boulevard, 99354, Richland, WA, USA aut Machida Masayuki Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, 062-8517, Sapporo, Hokkaido, Japan aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 3103-3113 0175-7598 99:7<3103 2015 99 253 https://doi.org/10.1007/s00253-014-6336-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6336-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tamano Koichi Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, 062-8517, Sapporo, Hokkaido, Japan aut NATIONALLICENCE 700 1- Bruno Kenneth Chemical and Biological Process Development Group, Pacific Northwest National Laboratory, 902 Battelle Boulevard, 99352, Richland, WA, USA aut NATIONALLICENCE 700 1- Koike Hideaki Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, 305-8566, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Ishii Tomoko Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, 062-8517, Sapporo, Hokkaido, Japan aut NATIONALLICENCE 700 1- Miura Ai Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, 062-8517, Sapporo, Hokkaido, Japan aut NATIONALLICENCE 700 1- Umemura Myco Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, 062-8517, Sapporo, Hokkaido, Japan aut NATIONALLICENCE 700 1- Culley David Chemical and Biological Process Development Group, Pacific Northwest National Laboratory, 902 Battelle Boulevard, 99352, Richland, WA, USA aut NATIONALLICENCE 700 1- Baker Scott Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, 3335 Innovation Boulevard, 99354, Richland, WA, USA aut NATIONALLICENCE 700 1- Machida Masayuki Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, 062-8517, Sapporo, Hokkaido, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 3103-3113 0175-7598 99:7<3103 2015 99 253