Potential of sequential treatment with minocycline and S. aureus specific phage lysin in eradication of MRSA biofilms: an in vitro study
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6460-1 |2 doi |
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| 245 | 0 | 0 | |a Potential of sequential treatment with minocycline and S. aureus specific phage lysin in eradication of MRSA biofilms: an in vitro study |h [Elektronische Daten] |c [Shivani Chopra, Kusum Harjai, Sanjay Chhibber] |
| 520 | 3 | |a Lysins are novel class of anti-infectives which are derived from bacteriophage. In the present study, the potential of previously characterised phage borne endolysin MR-10 in eradicating methicillin-resistant Staphylococcus aureus (MRSA) biofilm was evaluated. Scanning electron microscopic examination showed that both ica-positive and ica-negative MRSA formed equally potent mature biofilm. Different approaches were employed to eradicate the young as well as older biofilm formed by both types of MRSA strains. Our results showed a significant decrease (p < 0.01) in biofilm count on sequentially treating the MRSA biofilm with minocycline (4μg/ml) for 3h followed by treatment with endolysin MR-10. Since endolysin can act effectively irrespective of the metabolic status of the cells hence, they are capable of killing the rapidly growing cells (log phase cells) as well as non-dividing (stationary phase) cells. As a result they are effective in eradicating the younger and older biofilm. On staining the ica-positive MRSA biofilm with wheat germ agglutinin (WGA)-Alexa Flour 350, reduction in poly-intercellular adhesion (PIA) content was observed in comparison to control biofilm. In addition, a significant decrease (p < 0.01) in extracellular DNA (eDNA) content of ica-negative MRSA biofilm was also observed. Further, Live/Dead Baclight™ staining also showed presence of higher population of dead cells after treatment with minocycline and endolysin MR-10. Hence, our results showed that using minocycline sequentially with endolysin, MR-10 can effectively eradicate both young as well as older biofilm formed by ica-positive and ica-negative MRSA. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a MRSA |2 nationallicence | |
| 690 | 7 | |a Biofilm |2 nationallicence | |
| 690 | 7 | |a Endolysin |2 nationallicence | |
| 690 | 7 | |a Minocycline |2 nationallicence | |
| 700 | 1 | |a Chopra |D Shivani |u Department of Microbiology, Panjab University, 160014, Chandigarh, India |4 aut | |
| 700 | 1 | |a Harjai |D Kusum |u Department of Microbiology, Panjab University, 160014, Chandigarh, India |4 aut | |
| 700 | 1 | |a Chhibber |D Sanjay |u Department of Microbiology, Panjab University, 160014, Chandigarh, India |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/7(2015-04-01), 3201-3210 |x 0175-7598 |q 99:7<3201 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6460-1 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6460-1 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Chopra |D Shivani |u Department of Microbiology, Panjab University, 160014, Chandigarh, India |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Harjai |D Kusum |u Department of Microbiology, Panjab University, 160014, Chandigarh, India |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Chhibber |D Sanjay |u Department of Microbiology, Panjab University, 160014, Chandigarh, India |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/7(2015-04-01), 3201-3210 |x 0175-7598 |q 99:7<3201 |1 2015 |2 99 |o 253 | ||