caa a22 4500 605500215 CHVBK 20210128100554.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00253-015-6460-1 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6460-1 Potential of sequential treatment with minocycline and S. aureus specific phage lysin in eradication of MRSA biofilms: an in vitro study [Elektronische Daten] [Shivani Chopra, Kusum Harjai, Sanjay Chhibber] Lysins are novel class of anti-infectives which are derived from bacteriophage. In the present study, the potential of previously characterised phage borne endolysin MR-10 in eradicating methicillin-resistant Staphylococcus aureus (MRSA) biofilm was evaluated. Scanning electron microscopic examination showed that both ica-positive and ica-negative MRSA formed equally potent mature biofilm. Different approaches were employed to eradicate the young as well as older biofilm formed by both types of MRSA strains. Our results showed a significant decrease (p < 0.01) in biofilm count on sequentially treating the MRSA biofilm with minocycline (4μg/ml) for 3h followed by treatment with endolysin MR-10. Since endolysin can act effectively irrespective of the metabolic status of the cells hence, they are capable of killing the rapidly growing cells (log phase cells) as well as non-dividing (stationary phase) cells. As a result they are effective in eradicating the younger and older biofilm. On staining the ica-positive MRSA biofilm with wheat germ agglutinin (WGA)-Alexa Flour 350, reduction in poly-intercellular adhesion (PIA) content was observed in comparison to control biofilm. In addition, a significant decrease (p < 0.01) in extracellular DNA (eDNA) content of ica-negative MRSA biofilm was also observed. Further, Live/Dead Baclight™ staining also showed presence of higher population of dead cells after treatment with minocycline and endolysin MR-10. Hence, our results showed that using minocycline sequentially with endolysin, MR-10 can effectively eradicate both young as well as older biofilm formed by ica-positive and ica-negative MRSA. Springer-Verlag Berlin Heidelberg, 2015 MRSA nationallicence Biofilm nationallicence Endolysin nationallicence Minocycline nationallicence Chopra Shivani Department of Microbiology, Panjab University, 160014, Chandigarh, India aut Harjai Kusum Department of Microbiology, Panjab University, 160014, Chandigarh, India aut Chhibber Sanjay Department of Microbiology, Panjab University, 160014, Chandigarh, India aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 3201-3210 0175-7598 99:7<3201 2015 99 253 https://doi.org/10.1007/s00253-015-6460-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6460-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chopra Shivani Department of Microbiology, Panjab University, 160014, Chandigarh, India aut NATIONALLICENCE 700 1- Harjai Kusum Department of Microbiology, Panjab University, 160014, Chandigarh, India aut NATIONALLICENCE 700 1- Chhibber Sanjay Department of Microbiology, Panjab University, 160014, Chandigarh, India aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 3201-3210 0175-7598 99:7<3201 2015 99 253