caa a22 4500 605500231 CHVBK 20210128100555.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00253-014-6114-8 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6114-8 Characterization of a recombinant (+)-γ-lactamase from Microbacterium hydrocarbonoxydans which provides evidence that two enantiocomplementary γ-lactamases are in the strain [Elektronische Daten] [Jianjun Wang, Yaxin Zhu, Guogang Zhao, Junge Zhu, Sheng Wu] A two-step method, i.e., the transfer acyl analysis and then the chiral HPLC analysis, was employed in the screening of the cosmid library of Microbacterium hydrocarbonoxydans genome. Two enantiocomplementary γ-lactamase clones were found. A 40-kb cosmid showed (−)-γ-lactamase activity, and the activity was from Mhg which was reported previously according to the results of PCR identifying experiment. The 37-kb (+)-γ-lactamase cosmid was further constructed into a pUC18 plasmid library and screened by the same two-step method. A plasmid clone harboring a 1.6-kb fragment showed (+)-γ-lactamase activity. A 555-bp ORF in the 1.6-kb fragment showed high (+)-γ-lactamase activity when it was expressed under the control of T7 promoter. The coding protein showed significant homology with bacterial isochorismatase. The (+)-γ-lactamase was characterized and compared with the (−)-γ-lactamase Mhg. This is another report that two enantiocomplementary γ-lactamases are present in the same strain. Springer-Verlag Berlin Heidelberg, 2014 Cosmid library nationallicence Enantiocomplementary nationallicence γ-Lactamase nationallicence Isochorismatase nationallicence Wang Jianjun State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang, 100101, Beijing, People's Republic of China aut Zhu Yaxin State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang, 100101, Beijing, People's Republic of China aut Zhao Guogang Department of Internal Medicine, College of Medicine, University of Kentucky, 40536, Lexington, KY, USA aut Zhu Junge State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang, 100101, Beijing, People's Republic of China aut Wu Sheng State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang, 100101, Beijing, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 3069-3080 0175-7598 99:7<3069 2015 99 253 https://doi.org/10.1007/s00253-014-6114-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6114-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Jianjun State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang, 100101, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Zhu Yaxin State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang, 100101, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Zhao Guogang Department of Internal Medicine, College of Medicine, University of Kentucky, 40536, Lexington, KY, USA aut NATIONALLICENCE 700 1- Zhu Junge State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang, 100101, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Wu Sheng State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang, 100101, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 3069-3080 0175-7598 99:7<3069 2015 99 253