caa a22 4500 60550024X CHVBK 20210128100555.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00253-015-6414-7 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6414-7 Lactic acid fermentation within a cascading approach for biowaste treatment [Elektronische Daten] [Maraike Probst, Janette Walde, Thomas Pümpel, Andreas Wagner, Irene Schneider, Heribert Insam] Limited availability of resources and increased amounts of waste coupled with an ever-increasing demand for raw materials are typical characteristics of our times. As such, there is an urgent need to accordingly update waste treatment technology. The aim of this study was to determine whether a separate treatment of the liquid and the solid fraction of biowaste could enhance overall efficiency. Liquid fractions obtained from two different separation procedures were fermented at a pH of 5 and uncontrolled pH conditions for 72h. The fermentation conditions leading to highest lactic acid productivity and yield were evaluated. The substrates gained by both separation procedures showed efficient lactic acid production up to <25gL−1. The pH control increased lactic acid concentration by about 27%. Furthermore, quantitative real-time PCR analyses revealed stronger Lactobacilli growth in these fermentations. As identified via Illumina sequencing Lactobacillus delbrueckii and its closest relatives seemed to drive the fermentation independent of the substrate. These results could help to improve today's resourcing concept by providing a separate treatment of the liquid and solid biowaste fraction. Springer-Verlag Berlin Heidelberg, 2015 Municipal solid organic waste nationallicence Lactobacillus nationallicence Miseq Illumina sequencing nationallicence Biorefinery nationallicence Microbiome nationallicence Probst Maraike Institute of Microbiology, University of Innsbruck, Technikerstraße 25d, 6020, Innsbruck, Austria aut Walde Janette Department of Statistics, University of Innsbruck, Universitätsstraße 15, 6020, Innsbruck, Austria aut Pümpel Thomas Institute of Microbiology, University of Innsbruck, Technikerstraße 25d, 6020, Innsbruck, Austria aut Wagner Andreas Institute of Microbiology, University of Innsbruck, Technikerstraße 25d, 6020, Innsbruck, Austria aut Schneider Irene Unit of Environmental Engineering, University of Innsbruck, Technikerstraße 13, 6020, Innsbruck, Austria aut Insam Heribert Institute of Microbiology, University of Innsbruck, Technikerstraße 25d, 6020, Innsbruck, Austria aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 3029-3040 0175-7598 99:7<3029 2015 99 253 https://doi.org/10.1007/s00253-015-6414-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6414-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Probst Maraike Institute of Microbiology, University of Innsbruck, Technikerstraße 25d, 6020, Innsbruck, Austria aut NATIONALLICENCE 700 1- Walde Janette Department of Statistics, University of Innsbruck, Universitätsstraße 15, 6020, Innsbruck, Austria aut NATIONALLICENCE 700 1- Pümpel Thomas Institute of Microbiology, University of Innsbruck, Technikerstraße 25d, 6020, Innsbruck, Austria aut NATIONALLICENCE 700 1- Wagner Andreas Institute of Microbiology, University of Innsbruck, Technikerstraße 25d, 6020, Innsbruck, Austria aut NATIONALLICENCE 700 1- Schneider Irene Unit of Environmental Engineering, University of Innsbruck, Technikerstraße 13, 6020, Innsbruck, Austria aut NATIONALLICENCE 700 1- Insam Heribert Institute of Microbiology, University of Innsbruck, Technikerstraße 25d, 6020, Innsbruck, Austria aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 3029-3040 0175-7598 99:7<3029 2015 99 253