Optimization of volatile fatty acids and hydrogen production from Saccharina japonica: acidogenesis and molecular analysis of the resulting microbial communities

Verfasser / Beitragende:
[Kwonsu Jung, Woong Kim, Gwon Park, Charles Seo, Ho Chang, Yeu-Chun Kim]
Ort, Verlag, Jahr:
2015
Enthalten in:
Applied Microbiology and Biotechnology, 99/7(2015-04-01), 3327-3337
Format:
Artikel (online)
ID: 605500258
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024 7 0 |a 10.1007/s00253-015-6419-2  |2 doi 
035 |a (NATIONALLICENCE)springer-10.1007/s00253-015-6419-2 
245 0 0 |a Optimization of volatile fatty acids and hydrogen production from Saccharina japonica: acidogenesis and molecular analysis of the resulting microbial communities  |h [Elektronische Daten]  |c [Kwonsu Jung, Woong Kim, Gwon Park, Charles Seo, Ho Chang, Yeu-Chun Kim] 
520 3 |a Response surface methodology (RSM) was used to optimize the production of volatile fatty acids (VFAs) and hydrogen from mixed anaerobic cultures of Saccharina japonica with respect to two independent variables: methanogenic inhibitor concentration and temperature. The effects of four methanogenic inhibitors on acidogenic processes were tested, and qualitative microbial analyses were carried out. Escherichia, Acinetobacter, and Clostridium were the most predominant genera in samples treated with chloroform (CHCl3), iodoform (CHI3), 2-bromoethanesulfonate (BES), or β-cyclodextrin (β-CD), respectively. RSM showed that the production of VFAs reached a peak of 12.5g/L at 38.6°C in the presence of 7.4g/L β-CD; these were the conditions under which hydrogen production was also nearly maximal. The quantitative polymerase chain reaction (qPCR) showed that shifts in the bacterial community population correlated with the concentrations of β-CD indicating that this compound effectively inhibited methanogens. 
540 |a Springer-Verlag Berlin Heidelberg, 2015 
690 7 |a Response surface methodology  |2 nationallicence 
690 7 |a Volatile fatty acids  |2 nationallicence 
690 7 |a Acidogenesis  |2 nationallicence 
690 7 |a Anaerobic  |2 nationallicence 
690 7 |a Cyclodextrin  |2 nationallicence 
700 1 |a Jung  |D Kwonsu  |u Department of Chemical and Biomolecular Engineering, KAIST, 291, Daehak-ro, Yuseong-gu, 305-701, Daejeon, Republic of Korea  |4 aut 
700 1 |a Kim  |D Woong  |u Department of Chemical and Biomolecular Engineering, KAIST, 291, Daehak-ro, Yuseong-gu, 305-701, Daejeon, Republic of Korea  |4 aut 
700 1 |a Park  |D Gwon  |u Department of Chemical and Biomolecular Engineering, KAIST, 291, Daehak-ro, Yuseong-gu, 305-701, Daejeon, Republic of Korea  |4 aut 
700 1 |a Seo  |D Charles  |u Department of Chemical and Biomolecular Engineering, KAIST, 291, Daehak-ro, Yuseong-gu, 305-701, Daejeon, Republic of Korea  |4 aut 
700 1 |a Chang  |D Ho  |u Department of Chemical and Biomolecular Engineering, KAIST, 291, Daehak-ro, Yuseong-gu, 305-701, Daejeon, Republic of Korea  |4 aut 
700 1 |a Kim  |D Yeu-Chun  |u Department of Chemical and Biomolecular Engineering, KAIST, 291, Daehak-ro, Yuseong-gu, 305-701, Daejeon, Republic of Korea  |4 aut 
773 0 |t Applied Microbiology and Biotechnology  |d Springer Berlin Heidelberg  |g 99/7(2015-04-01), 3327-3337  |x 0175-7598  |q 99:7<3327  |1 2015  |2 99  |o 253 
856 4 0 |u https://doi.org/10.1007/s00253-015-6419-2  |q text/html  |z Onlinezugriff via DOI 
898 |a BK010053  |b XK010053  |c XK010000 
900 7 |a Metadata rights reserved  |b Springer special CC-BY-NC licence  |2 nationallicence 
908 |D 1  |a research-article  |2 jats 
949 |B NATIONALLICENCE  |F NATIONALLICENCE  |b NL-springer 
950 |B NATIONALLICENCE  |P 856  |E 40  |u https://doi.org/10.1007/s00253-015-6419-2  |q text/html  |z Onlinezugriff via DOI 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Jung  |D Kwonsu  |u Department of Chemical and Biomolecular Engineering, KAIST, 291, Daehak-ro, Yuseong-gu, 305-701, Daejeon, Republic of Korea  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Kim  |D Woong  |u Department of Chemical and Biomolecular Engineering, KAIST, 291, Daehak-ro, Yuseong-gu, 305-701, Daejeon, Republic of Korea  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Park  |D Gwon  |u Department of Chemical and Biomolecular Engineering, KAIST, 291, Daehak-ro, Yuseong-gu, 305-701, Daejeon, Republic of Korea  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Seo  |D Charles  |u Department of Chemical and Biomolecular Engineering, KAIST, 291, Daehak-ro, Yuseong-gu, 305-701, Daejeon, Republic of Korea  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Chang  |D Ho  |u Department of Chemical and Biomolecular Engineering, KAIST, 291, Daehak-ro, Yuseong-gu, 305-701, Daejeon, Republic of Korea  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Kim  |D Yeu-Chun  |u Department of Chemical and Biomolecular Engineering, KAIST, 291, Daehak-ro, Yuseong-gu, 305-701, Daejeon, Republic of Korea  |4 aut 
950 |B NATIONALLICENCE  |P 773  |E 0-  |t Applied Microbiology and Biotechnology  |d Springer Berlin Heidelberg  |g 99/7(2015-04-01), 3327-3337  |x 0175-7598  |q 99:7<3327  |1 2015  |2 99  |o 253