caa a22 4500 605500258 CHVBK 20210128100555.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00253-015-6419-2 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6419-2 Optimization of volatile fatty acids and hydrogen production from Saccharina japonica: acidogenesis and molecular analysis of the resulting microbial communities [Elektronische Daten] [Kwonsu Jung, Woong Kim, Gwon Park, Charles Seo, Ho Chang, Yeu-Chun Kim] Response surface methodology (RSM) was used to optimize the production of volatile fatty acids (VFAs) and hydrogen from mixed anaerobic cultures of Saccharina japonica with respect to two independent variables: methanogenic inhibitor concentration and temperature. The effects of four methanogenic inhibitors on acidogenic processes were tested, and qualitative microbial analyses were carried out. Escherichia, Acinetobacter, and Clostridium were the most predominant genera in samples treated with chloroform (CHCl3), iodoform (CHI3), 2-bromoethanesulfonate (BES), or β-cyclodextrin (β-CD), respectively. RSM showed that the production of VFAs reached a peak of 12.5g/L at 38.6°C in the presence of 7.4g/L β-CD; these were the conditions under which hydrogen production was also nearly maximal. The quantitative polymerase chain reaction (qPCR) showed that shifts in the bacterial community population correlated with the concentrations of β-CD indicating that this compound effectively inhibited methanogens. Springer-Verlag Berlin Heidelberg, 2015 Response surface methodology nationallicence Volatile fatty acids nationallicence Acidogenesis nationallicence Anaerobic nationallicence Cyclodextrin nationallicence Jung Kwonsu Department of Chemical and Biomolecular Engineering, KAIST, 291, Daehak-ro, Yuseong-gu, 305-701, Daejeon, Republic of Korea aut Kim Woong Department of Chemical and Biomolecular Engineering, KAIST, 291, Daehak-ro, Yuseong-gu, 305-701, Daejeon, Republic of Korea aut Park Gwon Department of Chemical and Biomolecular Engineering, KAIST, 291, Daehak-ro, Yuseong-gu, 305-701, Daejeon, Republic of Korea aut Seo Charles Department of Chemical and Biomolecular Engineering, KAIST, 291, Daehak-ro, Yuseong-gu, 305-701, Daejeon, Republic of Korea aut Chang Ho Department of Chemical and Biomolecular Engineering, KAIST, 291, Daehak-ro, Yuseong-gu, 305-701, Daejeon, Republic of Korea aut Kim Yeu-Chun Department of Chemical and Biomolecular Engineering, KAIST, 291, Daehak-ro, Yuseong-gu, 305-701, Daejeon, Republic of Korea aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 3327-3337 0175-7598 99:7<3327 2015 99 253 https://doi.org/10.1007/s00253-015-6419-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6419-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jung Kwonsu Department of Chemical and Biomolecular Engineering, KAIST, 291, Daehak-ro, Yuseong-gu, 305-701, Daejeon, Republic of Korea aut NATIONALLICENCE 700 1- Kim Woong Department of Chemical and Biomolecular Engineering, KAIST, 291, Daehak-ro, Yuseong-gu, 305-701, Daejeon, Republic of Korea aut NATIONALLICENCE 700 1- Park Gwon Department of Chemical and Biomolecular Engineering, KAIST, 291, Daehak-ro, Yuseong-gu, 305-701, Daejeon, Republic of Korea aut NATIONALLICENCE 700 1- Seo Charles Department of Chemical and Biomolecular Engineering, KAIST, 291, Daehak-ro, Yuseong-gu, 305-701, Daejeon, Republic of Korea aut NATIONALLICENCE 700 1- Chang Ho Department of Chemical and Biomolecular Engineering, KAIST, 291, Daehak-ro, Yuseong-gu, 305-701, Daejeon, Republic of Korea aut NATIONALLICENCE 700 1- Kim Yeu-Chun Department of Chemical and Biomolecular Engineering, KAIST, 291, Daehak-ro, Yuseong-gu, 305-701, Daejeon, Republic of Korea aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 3327-3337 0175-7598 99:7<3327 2015 99 253